3种方法吸附绿茶、黄茶与白茶香气成分的比较

doi:10.3785/j.issn.1008-9209.2019.09.191

浙江大学学报（农业与生命科学版）

2020, Vol. 46

Issue (4): 449-458 DOI: 10.3785/j.issn.1008-9209.2019.09.191

食品科学

3种方法吸附绿茶、黄茶与白茶香气成分的比较

陈娇娇¹(

),鲁成银²,王国庆²,陈利燕²,梁慧玲¹(

),张颖彬²(

)

^1.浙江农林大学农业与食品科学学院/浙江省农产品品质改良技术研究重点实验室，杭州 311300
^2.中国农业科学院茶叶研究所/农业农村部茶叶质量监督检验测试中心，杭州 310008

Comparison of three different methods for adsorption of aroma compounds for green tea, yellow tea and white tea

Jiaojiao CHEN¹(

),Chengyin LU²,Guoqing WANG²,Liyan CHEN²,Huiling LIANG¹(

),Yingbin ZHANG²(

)

^1.College of Agriculture and Food Science, Zhejiang A & F University/Zhejiang Provincial Key Laboratory of Agricultural Product Quality Improvement Technology, Hangzhou 311300, China
^2.Tea Research Institute, Chinese Academy of Agricultural Sciences/Tea Quality Inspection and Supervision Center, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China

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摘要：

结合气相色谱-质谱（gas chromatography-mass spectrometer, GC-MS）法，比较顶空固相微萃取（headspace solid-phase micro-extraction, HS-SPME）、顶空搅拌子吸附萃取（headspace stir bar sorptive extraction, HS-SBSE）、Tenax TA 3种方法吸附绿茶、黄茶与白茶香气成分的特点与规律，分析3种方法对供试的9个茶样香气成分的富集效果。结果表明：在相同的茶水比、平衡时间、萃取温度、吸附时间下，Tenax TA的吸附量最高，HS-SBSE其次，HS-SPME最低；HS-SPME与HS-SBSE对茶叶香气成分中的烯类物质和低水溶性物质有良好的富集能力；Tenax TA对醛类物质的富集效果更好，能捕集到部分易溶于水和极易挥发类物质。此外，HS-SPME和HS-SBSE吸附的芳樟醇及其氧化物和顺-己酸-3-己烯酯等长C链化合物的相对含量显著高于Tenax TA（P＜0.05），而2-甲基丁醛和1-戊烯-3-醇等短C链化合物的相对含量显著低于Tenax TA（P＜0.05）。考虑到HS-SPME与HS-SBSE吸附时存在一定的竞争效应，因此，Tenax TA吸附结果在反映香气化合物组成上更具真实性。

关键词： 顶空固相微萃取; 顶空搅拌子吸附萃取; Tenax TA吸附; 茶叶香气

Abstract:

Combined with gas chromatography-mass spectrometer (GC-MS), we compared the adsorption characteristics and laws of aroma compounds for green tea, yellow tea and white tea by using three methods, i.e. headspace solid-phase micro-extraction (HS-SPME), headspace stir bar sorptive extraction (HS-SBSE), and Tenax TA. Under the same ratio of tea to water, equilibrium time, extraction temperature and adsorption time, the enrichment effect of the three methods on aroma compounds for nine tea samples was analyzed. The results showed that the absorption capacity of Tenax TA was the highest; HS-SBSE was the second; and HS-SPME was the lowest. HS-SPME and HS-SBSE showed better enrichment effect for terpene and low water-soluble compounds, while Tenax TA showed better adsorption capacity of aldehyde and could trap some of the water-soluble and highly volatile substances easily. In addition, compared with Tenax TA, the relative content of long carbon chain compounds such as linalool, linalool oxide and cis-3-hexenyl hexanoate extracted by HS-SPME and HS-SBSE was significantly higher (P＜0.05), while the relative content of short carbon chain compounds including 2-methylbutyraldehyde and 1-penten-3-ol was lower (P＜0.05). In conclusion, the analysis results of Tenax TA method is more authentic in aroma composition, in consideration of a strong competition effect during HS-SPME and HS-SBSE extraction.

Key words: headspace solid-phase micro-extraction headspace stir bar sorptive extraction Tenax TA adsorption tea aroma

收稿日期: 2019-09-19 出版日期: 2020-09-11

CLC:

TQ 654

基金资助: 中国农业科学院科技创新工程(CAAS-ASTIP-2014-TRICAAS)

通讯作者: 梁慧玲,张颖彬 E-mail: 1639454215@qq.com;hlliang@zafu.edu.cn;yanying7217@sina.com

作者简介: 陈娇娇（https://orcid.org/0000-0001-7471-1014），E-mail：1639454215@qq.com

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引用本文:

陈娇娇,鲁成银,王国庆,陈利燕,梁慧玲,张颖彬. 3种方法吸附绿茶、黄茶与白茶香气成分的比较[J]. 浙江大学学报（农业与生命科学版）, 2020, 46(4): 449-458.

Jiaojiao CHEN,Chengyin LU,Guoqing WANG,Liyan CHEN,Huiling LIANG,Yingbin ZHANG. Comparison of three different methods for adsorption of aroma compounds for green tea, yellow tea and white tea. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(4): 449-458.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2019.09.191 或 http://www.zjujournals.com/agr/CN/Y2020/V46/I4/449

表1 3种方法吸附9个茶样香气成分的个数

表2 3种方法吸附9个茶样香气成分在3个C链范围内的个数

图1 3种方法吸附9个茶样香气成分的总峰面积HS-SPME：顶空固相微萃取；HS-SBSE：顶空搅拌子吸附萃取。短栅上不同小写字母表示同一茶样不同方法间在P＜0.05水平差异有统计学意义；n=3。

图2 3种方法吸附9个茶样香气成分的相对含量

图3 3种方法吸附9个茶样中C链香气成分的相对含量HS-SPME：顶空固相微萃取；HS-SBSE：顶空搅拌子吸附萃取。短栅上不同小写字母表示同一茶样不同方法间在P＜0.05水平差异有统计学意义；n=3。

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