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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2004, Vol. 30 Issue (4): 454-454    
论文     
Identification of novel Trichoderma hamatum genes expressed during mycoparasitism
Margaret Carpenter　 Alison Stewart　 Hayley Ridgway　
Margaret Carpenter（National Centre for Advanced Bio-Protection Technologies, PO Box 84, Lincoln University, Canterbury, New Zealand）　　　　　　Alison Stewart（National Centre for Advanced Bio-Protection Technologies, PO Box 84, Lincoln University, Canterbury, New Zealand）　　　　　　Hayley Ridgway（National Centre for Advanced Bio-Protection Technologies, PO Box 84, Lincoln University, Canterbury, New Zealand）　
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Abstract: Trichoderma species are currently used as biocontrol agents for crop diseases caused by a number of fungal plant pathogens. However, their biocontrol performance in the field can be unreliable and it is likely that more consistent performance could be achieved through knowledge and manipulation of the genes involved. For example, induction of the genes could be optimised for variable environmental and physiological conditions, superior strains could be selected more effectively and novel strains could be created. One method by which Trichoderma species accomplish biocontrol is mycoparasitism. Several genes involved in the mycoparasitic interaction have previously been characterised, however these consist predominantly of those that encode enzymes that degrade fungal cell walls. 　　In the current study subtractive hybridisation was used to target genes expressed when Trichoderma hamatum and the plant pathogen Sclerotinia sclerotiorum were cultured together, subtracting genes expressed when each are grown individually. This experimental design has the potential to yield T.hamatum genes involved in mycoparasitism of S. sclerotiorum, and S. sclerotiorum genes upregulated in host defence. The cDNA fragments yielded by the subtraction were characterised with respect to expression, sequence and species of origin. A number of novel T. hamatum genes which were up-regulated during mycoparasitism were identified.……
出版日期: 2004-07-12
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引用本文:

Margaret Carpenter　 Alison Stewart　 Hayley Ridgway　. Identification of novel Trichoderma hamatum genes expressed during mycoparasitism[J]. 浙江大学学报(农业与生命科学版), 2004, 30(4): 454-454.

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https://www.zjujournals.com/agr/CN/Y2004/V30/I4/454

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