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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2013, Vol. 39 Issue (2): 173-177    
论文     
辣椒叶提取物对α葡萄糖苷酶的抑制活性
李春英1*， 杨彦1， 李赫2， 李兰1， 励建荣3*
1.浙江大学生物系统工程与食品科学学院，杭州 310058；2.河南农业大学机电工程学院，郑州 450002；3.渤海大学食品科学研究院，辽宁 锦州 121013
Inhibitory effect of extract from Capsicum annuum leaves against αglucosidase activity. Journal of Zhejiang University
LI Chunying1*, YANG Yan1, LI He2, LI Lan1, LI Jianrong3*
1. School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; 2. College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China; 3. Research Institute of Food Science, Bohai University, Jinzhou, Liaoning 121013, China
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摘要： 收集不同辣椒品种，采用对硝基苯αD吡喃葡萄糖苷（pnitrophenylαDglucopyranoside, pNPG）对辣椒叶70%乙醇提取物进行α葡萄糖苷酶抑制活性测定，并通过动物糖负荷试验研究辣椒叶提取物的降糖效果. 结果表明：辣椒叶品系间对α葡萄糖苷酶抑制活性差异显著；同辣椒果实提取物相比，辣椒叶提取物对α葡萄糖苷酶抑制活性高达60%，是辣椒果实提取物的10倍左右；辣椒叶提取物对不同来源α葡萄糖苷酶表达不同，对于动物来源的蔗糖酶和麦芽糖酶的抑制具有不同程度的表达，而对于微生物来源的酵母（Saccharomyces cerevisiae）和嗜热芽孢杆菌（Bacillus stearothermophillus）的α葡萄糖苷酶抑制均没有表达；动物糖负荷试验表明，辣椒叶提取物具有较强的降血糖活性.
Abstract: Capsicum annuum is one of the world largest consumptive vegetables. As the biggest producer, consumer and exporter of C. annuum in the world, the development of China capsicum industry has an important influence on the development of global C. annuum industry. The C. annuum industry mostly focuses on its fruit, while C. annuum leaves, as a kind of easily wasted vegetables, which are rich in resources and nutrients, have not yet been widely developed. In order to better develop C. annuum leaves, we explore the αglucosidase inhibitory activity using 70% ethanol extracts of C. annuum leaves. The pnitrophenylαDglucopyranoside (pNPG) method was adopted to determine the inhibitory activity of C. annuum leaf extracts against αglucosidase. The results showed that the αglucosidase inhibitory activity of C. annuum leaf extracts was up to 60%, which was 10 times more than its fruit, and there were significant differences in the αglucosidase inhibitory activity among different cultivars. As to different target enzymes, C. annuum leaf extract showed different activity, for example, it could inhibit the activity of sucrase and maltase from animal, but it had no effect for microbial enzyme. The C. annuum leaf extract were validated with a strong hypoglycemic activity by animal glucose load test. In conclusion, The C. annuum leaf extract has high inhibition against αglucosidase and can be used for the development of a new type of hypoglycemic food.
出版日期: 2013-03-20
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李春英1*
杨彦1
李赫2
李兰1
励建荣3*

引用本文:

李春英1*， 杨彦1， 李赫2， 李兰1， 励建荣3*. 辣椒叶提取物对α葡萄糖苷酶的抑制活性[J]. 浙江大学学报(农业与生命科学版), 2013, 39(2): 173-177.

LI Chunying1*, YANG Yan1, LI He2, LI Lan1, LI Jianrong3*. Inhibitory effect of extract from Capsicum annuum leaves against αglucosidase activity. Journal of Zhejiang University. Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(2): 173-177.

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http://www.zjujournals.com/agr/CN/    或    http://www.zjujournals.com/agr/CN/Y2013/V39/I2/173

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