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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2004, Vol. 30 Issue (4): 443-443    
论文     
Study on culturing Trichodema mutants
CHEN Jian-ai　 WANG Wei-ming　
CHEN Jian-ai（Institute for Application of Atomic Energy, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China）　　　　　　
WANG Wei-ming（Institute for Application of Atomic Energy, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China）
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Abstract: Trichodema mutants strains T5, T0803, T1010, T1003were cultured in different conditions and media, also in the presence of fungicides at 40 mg/kg (CK or procymidone + chlorothalonil, or maneb or phosethyl-Al) . The pH values of media were 5, 6, 7 and 8 and hyphae were grown at temperatures of 15, 20, 25 and 30 ℃. After being cultured for 3, 4, 5, or 6 days, the strains were transferred at a lower temperature to sporulate (20℃) Obtained data were analyzed statistically, with the orthogonal array and ranges (R) differing dependes on the treatments (R = 40.0,42.4, 48.0, 62.8,107.0). The results indicated that the most important factor was the nature of the strain (R =107.0), while the change in temperature and time of cultivation produced the lowest effect (R =40.0). Each factor variance was significant and A3B4C2D1E3 was the optimum combined condition, in which strain T1010 grew more quickly and sporulated most.……
出版日期: 2004-07-12
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引用本文:

CHEN Jian-ai　 WANG Wei-ming　. Study on culturing Trichodema mutants[J]. 浙江大学学报(农业与生命科学版), 2004, 30(4): 443-443.

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https://www.zjujournals.com/agr/CN/Y2004/V30/I4/443

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