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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2004, Vol. 30 Issue (4): 442-442    
论文     
Genetic regulation of conidiation in Trichoderma hamatun
Johanna Steyaert　 Travis Glare　 Alison Stewart　 Margaret Carpenter　 Hayley Ridgway　
Johanna Steyaert（National Centre for Advanced Bio-Protection Technologies, PO Box 84, Lincoln University, New Zealand）　　　　　　
Travis Glare（AgResearch Ltd., PO Box 60, Lincoln, New Zealand）　　　　　　
Alison Stewart（National Centre for Advanced Bio-Protection Technologies, PO Box 84, Lincoln University, New Zealand）　　　　　　
Margaret Carpenter（National Centre for Advanced Bio-Protection Technologies, PO Box 84, Lincoln University, New Zealand）　　　　　　
Hayley Ridgway（National Centre for Advanced Bio-Protection Technologies, PO Box 84, Lincoln University, New Zealand）
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Abstract: Achieving a balance between vegetative growth and spore production is essential for successful biocontrol by fungi. Low sporulation rates in the field can result in poor establishment and survival,whereas failure of conidia to recognise hosts can lead to persistence without efficacy. Commercial biocontrol products involve bulk preparations of conidia, however considerable variability in conidiation rates exists between biocontrol agents, which can restrict choice of strain for production. The majority of studies on Trichoderma conidiation have focused on the species T. viride and T. atroviride.These species form conidia in response to blue and near-UV light and/or nutrient deprivation and conidiation proceeds in a highly co-ordinated fashion, however relatively little is known on the genetic basis of Trichoderrma conidiation. In addition, whilst photoconidiation appears to be a general response detailed studies in other Trichoderma species are absent. In this study, conidiation in the lesser known biocontrol species T. hamatum is being investigated using a combined morphological and molecular approach. In contrast to T. atroviride, conidiation in response to blue-light was weaker and variable and suggested that additional triggers may be required for the T. hamatum photoresponse. A series of comparative photoconidiation assays are currently being undertaken investigating the effect of inoculum type and abiotic factors on timing and intensity of the response.Results will be discussed in relation to the current knowledge on conidial morphogenesis in Trichoderma. In addition to these morphological assays, a selection of genes implicated in sporulation and the blue-light responses are currently being isolated and characterised from T. hamatum. Two genes, phr1 and cmp1 , which were isolated previously from T. atroviride will be used as early and late markers of gene expression during the photoresponse in T. hamatum in order to define time points for harvesting comparable stage-specific RNA from T. hamatum and T. atroviride. Using degenerate PCR putative sporulation gene orthologues have also been identified in T. hamatum.Work is currently underway to isolate genomic clones of these genes from T. hamatum and T.atroviride. Sequence and expression analysis of orthologues, including expression in response to abiotic factors will be presented and discussed in relation to the current knowledge of the molecular basis of conidiation in Trichoderma and other filamentous fungi.……
出版日期: 2004-07-12
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Johanna Steyaert　 Travis Glare　 Alison Stewart　 Margaret Carpenter　 Hayley Ridgw. Genetic regulation of conidiation in Trichoderma hamatun[J]. 浙江大学学报(农业与生命科学版), 2004, 30(4): 442-442.

链接本文:

https://www.zjujournals.com/agr/CN/Y2004/V30/I4/442

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