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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2004, Vol. 30 Issue (4): 436-436    
论文     
Xylanase production by Trichoderma strains in solid substrate fermentation
Krisztina Kovacs，George Szakacs， Lew Christopher
Krisztina Kovacs(D epartmento fA griculturalC hemicalT echnology， TechnicalU niversityo fB udapests，1111B udapest，Gellertte r4 ，Hungary) George Szakacs(D epartmento fA griculturalC hemicalT echnology， TechnicalU niversityo fB udapests，1111B udapest，Gellertte r4 ，Hungary) Lew Christopher(Sappi Biotechnology Laboratory，Department of Microbiology，Biochemistry and Food Sciences，University of the Free State，9300 Bloemfontein，South Africa and Sappi Forest Products Technology Centre，Sappi Management Services，PO Box 3252，1560 Springs，South Africa)
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Abstract: The importance of microbial enzymes in pulp and paper manufacturing has grown significantly in the last two decades. Solid substrate fermentation (SSF) holds tremendous potential for the production of microbial enzymes of commercial interest. SSF can be of special interest in those processes where the crude fermented product (whole SSF culture, in situ enzyme) may be used directly as the enzyme source. Xylanase preparations practically free of cellulase activity are especially useful for biobleaching of crude cellulose pulps. Thirty-nine Trichoderma isolates have been screened in SSF for xylanase production on hardwood oxygen-delignified soda-aq pulp as carbon source and enzyme inducer.Xylanase activities varied between 0 and 2200 IU/g dry matter (DM) of initial substrate. In most instances, the simultaneously produced cellulase levels were below 1.0 Filter Paper Unit (FPU) /g DM. The xylanase to cellulase activity ratio varied in the range of 5 to 3500. The three most promising isolates (TUB F-1647, TUB F-1658 and TUB F-1684) yielded xylanase activity of 2040,1300 and 1500 IU/g DM xylanase, respectively, and 0.64, 0.43 and 0.43 FPU/g DM cellulase with a xylanase to cellulase activity ratio of 3200, 3000 and 3500, respectively. Wild strains F-1647, F-1658 and F-1684 were isolated from tree bark of Maldives, soils of Peru (last two), respectively.Medium optimization experiments to enhance the xylanase yield and to increase the xylanase to cellulase ratio have also been performed.……
出版日期: 2004-07-12
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Krisztina Kovacs，George Szakacs， Lew Christopher. Xylanase production by Trichoderma strains in solid substrate fermentation[J]. 浙江大学学报(农业与生命科学版), 2004, 30(4): 436-436.

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https://www.zjujournals.com/agr/CN/Y2004/V30/I4/436

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