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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2024, Vol. 50 Issue (5): 667-678    DOI: 10.3785/j.issn.1008-9209.2023.11.271
青年科学家论坛     
植物过氧化物酶体的代谢功能和蛋白质组成研究进展
张钰婵1(),戴华鑫2,3(),潘荣辉1()
1.浙江大学农业与生物技术学院现代种业研究所, 浙江 杭州 310058
2.中国烟草总公司郑州烟草研究院, 河南 郑州 450001
3.北京生命科技研究院, 北京 102209
Research progress on the metabolic functions and protein compositions of plant peroxisomes
Yuchan ZHANG1(),Huaxin DAI2,3(),Ronghui PAN1()
1.Advanced Seed Institute, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China
2.Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou 450001, Henan, China
3.Beijing Life Science Academy, Beijing 102209, China
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摘要：

过氧化物酶体是真核生物中普遍存在的多功能细胞器。在植物中，过氧化物酶体具有极其丰富的初级和次级代谢功能，在植物的生长发育和抗逆反应中发挥重要作用。因此，植物过氧化物酶体研究对提高农作物产量、品质和抗逆性都有重要意义。过氧化物酶体的代谢功能依赖于过氧化物酶体定位的蛋白质，包括各种酶和负责物质运输的膜蛋白，因此，揭示过氧化物酶体蛋白组成有利于了解其代谢功能。本文总结了植物过氧化物酶体的主要代谢功能，以及有关植物过氧化物酶体的蛋白质组研究，并对植物过氧化物酶体未来研究方向及其与农业的关系进行了展望。
关键词： 过氧化物酶体;  植物;  代谢功能;  蛋白质组    
Abstract:

Peroxisomes are multifunctional organelles that are ubiquitous in eukaryotes. In plants, peroxisomes have very diverse functions in primary and secondary metabolisms and play important roles in plant growth and development as well as in stress response. Therefore, the study of plant peroxisomes is of great significance for improving crop yield, quality and stress resistance. The metabolic functions of peroxisomes depend on peroxisome-localized proteins, including various enzymes and membrane proteins responsible for substance transport; thus revealing the protein composition of peroxisomes is beneficial for understanding their metabolic functions. This paper summarized the main metabolic functions of plant peroxisomes and the proteomic studies of plant peroxisomes. Furthermore, we concluded with an outlook on future research directions related to plant peroxisomes and their relationship with agriculture.
Key words: peroxisomes    plant    metabolic functions    proteome
收稿日期: 2023-11-27 出版日期: 2024-10-31
CLC:  Q734  
基金资助: 中国工程科技发展战略河南研究院战略咨询研究项目(2023HENZDB01);中国烟草总公司首席科学家创新专项(112023CK0820);北京生命科技研究院有限公司科技项目(2023000CC0010);国家自然科学基金项目(32200231);浙江省自然科学基金项目(LZ23C020002);国家重点研发计划项目(2022YFD1401600)
通讯作者: 戴华鑫,潘荣辉     E-mail: 11716038@zju.edu.cn;daihx@ztri.com.cn;panr@zju.edu.cn
作者简介: 张钰婵（https://orcid.org/0000-0002-3509-6597），E-mail：11716038@zju.edu.cn|潘荣辉 ，浙江大学“百人计划”研究员，博士生导师，浙江大学杭州国际科创中心“科创百人”研究员，浙江大学现代种业研究所副所长。2009年本科毕业于南京大学，2014年博士毕业于密歇根州立大学。2018年入职浙江大学，从事作物细胞代谢研究，系统解析作物能量细胞器代谢网络，重点挖掘作物能量细胞器逆境代谢和种子代谢功能。以通信作者在Trends in Biotechnology、Developmental Cell、The Plant Journal、Journal of Integrative Plant Biology、The Crop Journal、aBiotech 等期刊发表论文，并以第一作者在 PNAS、The Plant Cell、New Phytologist、Journal of Integrative Plant Biology、The Plant Journal 等期刊发表论文，参与出版《种子学》《植物生物技术》等教材，以及Subcellular Biochemistry、Methods in Photorespiration等专著。近年来入选浙江大学“仲英青年学者”、浙江大学杭州国际科创中心“青年人才卓越计划”、浙江省“领军型创新团队”等。担任Crop Design期刊编委。课题组博士后先后获得“博新计划”、中国博士后科学基金面上资助、国际引进交流计划等国家级博士后项目支持。欢迎优秀博士加入本团队。（https://orcid.org/0000-0002-4264-5566），E-mail：panr@zju.edu.cn
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引用本文:

张钰婵,戴华鑫,潘荣辉. 植物过氧化物酶体的代谢功能和蛋白质组成研究进展[J]. 浙江大学学报（农业与生命科学版）, 2024, 50(5): 667-678.

Yuchan ZHANG,Huaxin DAI,Ronghui PAN. Research progress on the metabolic functions and protein compositions of plant peroxisomes. Journal of Zhejiang University (Agriculture and Life Sciences), 2024, 50(5): 667-678.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2023.11.271    或    https://www.zjujournals.com/agr/CN/Y2024/V50/I5/667

图1  植物过氧化物酶体的代谢功能
图2  光呼吸代谢途径示意图黑色粗箭头代表光呼吸的核心代谢反应。Rubisco：核酮糖-1，5-二磷酸羧化酶/加氧酶；PGLP：2-磷酸乙醇酸磷酸酶；GLYK：甘油酸激酶；GOX：乙醇酸氧化酶；HPR1/2：羟基丙酮酸还原酶1/2；CAT2：过氧化氢酶2；GGT：谷氨酸：乙醛酸氨基转移酶；SGT：丝氨酸：乙醛酸氨基转移酶；GDC：甘氨酸脱羧酶复合体；SHMT：丝氨酸羟甲基转移酶；？表示可能存在的未知转运蛋白。
图3  植物过氧化物酶体的活性氧清除机制MDA：单脱氢抗坏血酸；ASC：抗坏血酸；APX3：抗坏血酸过氧化物酶3；MDAR1/4：单脱氢抗坏血酸还原酶1/4；CAT1/2/3：过氧化氢酶1/2/3；DHA：脱氢抗坏血酸；NAD＋：烟酰胺腺嘌呤二核苷酸；NADH：还原型烟酰胺腺嘌呤二核苷酸；DHAR1：脱氢抗坏血酸还原酶1；GSTT1：谷胱甘肽巯基转移酶θ1；GSH：谷胱甘肽；GSSG：氧化型谷胱甘肽；GR1：谷胱甘肽还原酶1；NADP＋：烟酰胺腺嘌呤二核苷酸磷酸；NADPH：还原型烟酰胺腺嘌呤二核苷酸磷酸。
图4  植物过氧化物酶体蛋白引入机制PTS：过氧化物酶体靶向信号；PMP：过氧化物酶体膜蛋白。
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