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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2024, Vol. 50 Issue (5): 715-723    DOI: 10.3785/j.issn.1008-9209.2023.06.272
综述     
组蛋白乙酰化调控因子在动物早期胚胎发育过程中的作用研究进展
李潇腾(),赵盼盼,张坤()
浙江大学动物科学学院，浙江省奶牛遗传改良与乳品质研究重点实验室，浙江 杭州 310058
Research progress on the role of histone acetylation regulatory factors during the early embryonic development in animals
Xiaoteng LI(),Panpan ZHAO,Kun ZHANG()
Key Laboratory of Dairy Cow Genetic Improvement and Milk Quality Research of Zhejiang Province, College of Animal Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China
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摘要：

精卵结合后，动物胚胎在早期发育过程中会经历剧烈的表观遗传重编程，包括DNA甲基化、组蛋白修饰和染色质重塑等，以确保受精前后高度分化的配子形成全能性的细胞。重编程与早期胚胎发育过程的各个阶段紧密相关，有多种调控因子参与其中，如乙酰转移酶、脱乙酰酶及组蛋白修饰阅读器等均参与组蛋白乙酰化的调控，并发挥重要功能。本文综述了组蛋白乙酰化调控因子在动物早期胚胎发育过程中的作用，探讨了早期胚胎发育过程中的表观遗传现象及机制，有助于更好地理解哺乳动物早期胚胎发育机制，减少胚胎发育缺陷，从而提升家畜繁殖力。
关键词： 家畜;  繁殖;  早期胚胎;  着床前;  表观遗传;  组蛋白乙酰化    
Abstract:

Following sperm-egg fusion, animal embryos undergo severe epigenetic reprogramming during the early development, encompassing DNA methylation, histone modification, as well as chromatin remodeling, to ensure that highly differentiated gametes form totipotent embryonic cells upon fertilization. Reprogramming is intimately connected to each stage of early embryonic development, and numerous regulatory factors are involved, such as acetyltransferases, deacetylases and histone modification readers, which participate in the regulation of histone acetylation and serve crucial functions. This review summarizes the roles of histone acetylation regulatory factors in the early embryonic development of animals, discusses epigenetic phenomena and mechanisms during the early embryonic development, contributes to a better understanding of the developmental mechanisms of mammalian early embryos, reduces embryonic developmental defects, and enhances livestock fertility.
Key words: domestic animal    fertility    early embryo    preimplantation    epigenetics    histone acetylation
收稿日期: 2023-06-27 出版日期: 2024-10-31
CLC:  S813  
基金资助: 浙江省农业（畜禽）新品种选育重大科技专项(2021C02068-1-3);国家自然科学基金国际（地区）合作与交流项目(32161143032)
通讯作者: 张坤     E-mail: xxiaotl@163.com;kzhang@zju.edu.cn
作者简介: 李潇腾（https://orcid.org/0009-0007-2790-8157），E-mail：xxiaotl@163.com
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李潇腾
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引用本文:

李潇腾,赵盼盼,张坤. 组蛋白乙酰化调控因子在动物早期胚胎发育过程中的作用研究进展[J]. 浙江大学学报（农业与生命科学版）, 2024, 50(5): 715-723.

Xiaoteng LI,Panpan ZHAO,Kun ZHANG. Research progress on the role of histone acetylation regulatory factors during the early embryonic development in animals. Journal of Zhejiang University (Agriculture and Life Sciences), 2024, 50(5): 715-723.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2023.06.272    或    https://www.zjujournals.com/agr/CN/Y2024/V50/I5/715

图1  哺乳动物早期胚胎发育与表观遗传重编程左边图组为一般哺乳动物早期胚胎发育阶段模式图；右边图组为表观遗传重编程的3个主要过程和对应的染色质结构变化。Me：甲基化。
图2  组蛋白乙酰化及其相关因子在哺乳动物早期胚胎发育中的作用组蛋白氨基末端特定的赖氨酸残基在组蛋白乙酰转移酶的作用下被乙酰基修饰，发生组蛋白乙酰化，常见的乙酰转移酶包括SRC-3、PCAF、CBP/P300（作用于转录调控和正常ZGA）、TIP60（参与转录调节和DNA损伤修复）和GCN5（维持细胞周期和基因组完整）等；乙酰化赖氨酸残基被乙酰化修饰阅读器识别并结合，如BRD4蛋白参与ZGA激活、X染色体上调启动及调控NANOG表达；在组蛋白脱乙酰酶作用下发生去乙酰化，常见的脱乙酰酶有HDAC1/2（维持正常ZGA和胚胎发育）、SIN3A（通过CCNB1调控猪胚胎，通过HDAC1调控小鼠胚胎）、RBBP4/7（促进细胞周期进程与谱系发育）、SUDS3（通过FGF4/ERK通路影响谱系特化）、NuRD和NcoR等。组蛋白乙酰化动态过程与转录活性均密切相关。Ac：乙酰化。
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