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Species diversity of Lachnum (Helotiales, Hyaloscyphaceae) from temperate China
Ye Ming, Cao Shu-qing, Jiang Shao-tong, Pan Li-jun, Luo Shui-zhong, Li Xing-jiang
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(1): 4-.
https://doi.org/10.1631/jzus.2006.B0020
Twenty-three temperate China species of Lachnum, Lachnum abnorme, L. angustum, L. brevipilosum, L. calosporum, L. calyculiforme, L. carneolum, L. ciliare, L. controversum, L. flavidulum, L. cf. fushanese, L. indicum, L. kumaonicum, L. lushanese, L. minutum, L. montanum, L. cf. pteridophyllum, L. pygmaeum, L. sclerotii var. sclerotii, L. sclerotii var. sichuanense, L. subpygmeaum, L. tenuissimum, L. virgineum and L. willisii are reported, whose main characteristics are given in a formula of the described species, some of which are discussed below.
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Promoter trapping in Magnaporthe grisea
Liu Xiao-hong, Lu Jian-ping, Wang Jiao-yu, Min Hang, Lin Fu-cheng
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(1): 5-.
https://doi.org/10.1631/jzus.2006.B0028
Application of promoter trapping based on transformation in Magnaporthe grisea is reported in this paper. Two promoter-trapping vectors, designated as pCBGFP and pEGFPHPH, were constructed and transformed into protoplasts of M. grisea. A library of 1077 transformants resistant to hygromycin B was generated. Of which, 448 transformants were found to express eGFP gene in different structures of M. grisea. Three transformants grew slowly, 5 transformants decreased in conidiation and 7 transformants reduced in pathogenicity greatly among these 448 transformants. Eleven transformants were checked by genomic southern blot randomly, and 9 of which were single-copy insertions. The promoter trapping technique has been applied successfully in M. grisea and can be used as a tool for functional genomic analysis.
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Field demonstration of reduction of lead availability in soil and cabbage (Brassica Chinensis L.) contaminated by mining tailings using phosphorus fertilizers
Xie Zheng-miao, Wang Bi-ling, Sun Ye-fang, Li Jing
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(1): 8-.
https://doi.org/10.1631/jzus.2006.B0043
A field demonstration of reduction of lead availability in a soil and cabbage (Brassica Chinensis L.) contaminated by mining tailings, located in Shaoxing, China was carried out to evaluate the effects of applications of phosphorus fertilizers on Pb fractionation and Pb phytoavailability in the soil. It was found that the addition of all three P fertilizers including single super phosphate (SSP), phosphate rock (PR), and calcium magnesium phosphate (CMP) significantly decreased the percentage of water-soluble and exchangeable (WE) soil Pb and then reduced the uptake of Pb, Cd, and Zn by the cabbage compared to the control (CK). The results showed that the level of 300 g P/m2 soil was the most cost-effective application rate of P fertilizers for reducing Pb availability at the first stage of remediation, and that at this P level, the effect of WE fraction of Pb in the soil decreased by three phosphorus fertilizers followed the order: CMP (79%)>SSP (41%)>PR (23%); Effectiveness on the reduction of Pb uptake by cabbage was in the order: CMP (53%)>SSP (41%)>PR (30%). Therefore our field trial demonstrated that it was effective and feasible to reduce Pb availability in soil and cabbage contaminated by mining tailings using P fertilizers in China and PR would be a most cost-effective amendment.
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Bread wheat varieties as influenced by different nitrogen levels
Hussain Iqtidar, Khan Muhammad Ayyaz, Khan Ejaz Ahmad
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(1): 12-.
https://doi.org/10.1631/jzus.2006.B0070
Experiment was conducted to determine the effect of different nitrogen levels on four bread wheat varieties (Triticum aestivum L.) viz. Inqilab-91, Daman-98, Dera-98 and Punjab-96 at Gomal University, Dera Ismail Khan (NWFP), Pakistan during 2000~2001. The experiment was laid out in split plot design having four replications using a net plot size of 2 m×5 m. Nitrogen doses used were 0, 50, 100, 150 and 200 kg/ha. The results showed that different nitrogen levels had significant effects on plant height, total number of plants/m2, number of grains/spike, number of spike/m2, spike weight, biological yield, grain yield and grain protein content. Maximum plant height, total number of plants/m2, number of spikes/m2, spike weight, biological yield and grain protein content were observed at 200 kg N/ha. Among wheat varieties Daman-98 had maximum plant height, spike weight, grains/spike, 1000-grain weight, biological yield and grain yield. Inqilab-91 had heavier grains and the most grain protein content, while Dera-98 had the maximum plant population and spikes/m2. Grain yield and biological yield were statistically similar at doses of 150 kg N/ha and 200 kg N/ha. However, dose of 200 kg N/ha, compared to dose of 150 Kg N/ha, significantly increased the protein content.
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Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system
Gong Xing-guo, Ji Jing, Xie Jie, Zhou Yuan, Zhang Jun-yan, Zhong Wen-tao
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(1): 13-19.
https://doi.org/10.1631/jzus.2006.B0013
v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST (glutathione S-transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl β-D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression.
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Nutritional quality, functional properties and anti-nutrient compositions of the larva of Cirina forda (Westwood) (Lepidoptera: Saturniidae)
Omotoso O.T.
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(1): 51-55.
https://doi.org/10.1631/jzus.2006.B0051
Determination of the proximate, nutritionally valuable minerals and anti-nutrient compositions in larvae of Cirina forda (W) showed that they contained high levels of protein, (55.50%±1.20%) with ash, moisture, fat, and carbohydrate levels being (10.26%±0.01%), (10.85%±0.38%), (4.68%±0.01%) and (18.70%±0.84%) respectively. Fibre was not detected. Protein solubility was higher in alkaline media than in acidic media while the isoelectric points were pH 4, 6 and 9 indicating that C. forda may be useful in food formulations involving foods like meat products. Phosphorus had the highest value of (215.54±0.21) mg/100 g while manganese had the lowest value of (1.14±0.10) mg/100 g. Copper, Co, Pb, Cr and Ni were not available. Water absorption capacity was (300%±0.15%), oil absorption capacity was (358.44%±0.21%) and foaming stability was (3%±0.00%). The results of anti-nutritional analysis revealed that oxalate and phytic acid contents were (4.11±0.05) mg/100 g and (1.02±0.00) mg/100 g respectively and that these values fell within nutritionally accepted values. Tannin was not detected in C. forda. C. forda, a rich source of animal nutrients, usable in human diets and animal feeds formulations.
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Structural changes of oviduct of freshwater shrimp, Macrobrachium nipponense (Decapoda, Palaemonidae), during spawning
Lu Jian-ping, Zhang Xiao-hui, Yu Xiao-yun
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(1): 64-69.
https://doi.org/10.1631/jzus.2006.B0064
The structural change of the oviduct of freshwater shrimp (Macrobrachium nipponense) during spawning was examined by electron microscopy. The oviduct wall structural characteristics seem to be influenced significantly by the spawning process. Before the parturition and ovulation, two types of epithelial cells (types I and II) are found in the epithelium. The free surfaces of type I and type II cells have very dense long microvilli. Under the type I and type II cells, are a relatively thick layer of secreting material and a layer of mostly dead cells. After ovulation, two other types of epithelial cells (types III and IV) are found in the oviduct wall epithelium. The free surface of type III cells only has short microvilli scattered on the surface. The thick layer with secreting material and the dead cell layer disappeared at this stage. In some type III cells, the leaking out of cytoplasm from broken cell membrane led to the death of these type III cells. The transformation of all four types of epithelial cells was in the order: IV→I→II→III.
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13 articles
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