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Acute phase reactants, challenge in the near future of animal production and veterinary medicine
GRUYS E., TOUSSAINT M.J.M., UPRAGARIN N., VAN EDEREN A.M., ADEWUYI A.A., CANDIANI D., NGUYEN T.K.A., SABECKIENE (BALCIUTE) J.
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(10): 1-.   https://doi.org/10.1631/jzus.2005.B0941
Abstract( 2871 )     PDF(0KB)( 1057 )
The future of acute phase proteins (APPs) in science is discussed in this paper. Many functions and associated pathological processes of APPs are unknown. Extrahepatic formation in local tissues needs attention. Local serum amyloid A (SAA) formation may be involved in deposition of AA-amyloid induced by conformational change of SAA resulting in amyloid formation, having tremendous food safety implications. Amyloidogenesis is enhanced in mouse fed beta pleated sheet-rich proteins. The local amyloid in joints of chicken and mammary corpora amylacea is discussed. Differences in glycosylation of glycoproteins among the APPs, as has been shown for α1-acid glycoprotein, have to be considered. More knowledge on the reactivity patterns may lead to implication of APPs in the diagnostics and staging of a disease. Calculation of an index from values of several acute phase variables increases the power of APPs in monitoring unhealthy individuals in animal populations. Vaccinations, just as infections in eliciting acute phase response seem to limit the profitability of vaccines because acute phase reactions are contraproductive in view of muscle anabolism. Interest is focused on amino acid patterns and vitamins in view of dietary nutrition effect on sick and convalescing animals. When inexpensive methodology such as liquid phase methods (nephelometry, turbidimetry) or protein array technology for rapid APP measurement is available, APPs have a future in routine diagnostics. Specific groups of patients may be screened or populations monitored by using APP.
Dependence of nucleotide physical properties on their placement in codons and determinative degree
BEREZHNOY A.Y., DUPLIJ S.A.
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(10): 2-.   https://doi.org/10.1631/jzus.2005.B0948
Abstract( 2197 )     PDF(0KB)( 1038 )
Various physical properties such as dipole moment, heat of formation and energy of the most stable formation of nucleotides and bases were calculated by PM3 (modified neglect of diatomic overlap, parametric method number 3) and AM1 (austin model 1) methods. As distinct from previous calculations, for nucleotides the interaction with neighbours is taken into account up to gradient of convergence equaling 1. The dependencies of these variables from the place in the codon and the determinative degree were obtained. The difference of these variables for codons and anticodons is shown.
Parameters selection in gene selection using Gaussian kernel support vector machines by genetic algorithm
MAO Yong, ZHOU Xiao-bo, PI Dao-ying, SUN You-xian, WONG Stephen T.C.
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(10): 3-.   https://doi.org/10.1631/jzus.2005.B0961
Abstract( 3181 )     PDF(0KB)( 1238 )
In microarray-based cancer classification, gene selection is an important issue owing to the large number of variables and small number of samples as well as its non-linearity. It is difficult to get satisfying results by using conventional linear statistical methods. Recursive feature elimination based on support vector machine (SVM RFE) is an effective algorithm for gene selection and cancer classification, which are integrated into a consistent framework. In this paper, we propose a new method to select parameters of the aforementioned algorithm implemented with Gaussian kernel SVMs as better alternatives to the common practice of selecting the apparently best parameters by using a genetic algorithm to search for a couple of optimal parameter. Fast implementation issues for this method are also discussed for pragmatic reasons. The proposed method was tested on two representative hereditary breast cancer and acute leukaemia datasets. The experimental results indicate that the proposed method performs well in selecting genes and achieves high classification accuracies with these genes.
Effect of cadmium on growth and photosynthesis of tomato seedlings
DONG Jing, WU Fei-bo, ZHANG Guo-ping
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(10): 4-.   https://doi.org/10.1631/jzus.2005.B0974
Abstract( 3250 )     PDF(0KB)( 1177 )
A hydroponic experiment carried out to study the effect of five Cd levels on growth and photosynthesis of two tomato cultivars showed that the addition of 0.1 μmol/L Cd induced a slight increase in plant height of Hezuo 903 and the SPAD (the Soil–Plant Analyses Development) value of the 2 cultivars. However, at higher Cd levels, i.e., 1 and 10 μmol/L, root length and volume, plant height, and SPAD value were all significantly reduced. On an average of the 2 cultivars, exposure to 1 and 10 μmol/L Cd for 33 d reduced plant height by 18.9% and 46.4% and SPAD value by 11.2% and 31.6%, compared with control, respectively. Similarly, root length was reduced by 41.1% and 25.8% and root volume by 45.2% and 63.7%, respectively. The addition of Cd in the growth medium also had significant deleterious effect on net photosynthetic rate (Pn) and intracellular CO2 concentration (Ci), with Pn being reduced by 27.2% and 62.1% at 1 μmol/L and 10 μmol/L Cd treatments compared to the control, respectively, while Ci increased correspondingly by 28.4% and 39.3%.
Identification of technology options for reducing nitrogen pollution in cropping systems of Pujiang
FANG Bin, WANG Guang-huo, VAN DEN BERG Marrit, ROETTER Reimund
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(10): 5-.   https://doi.org/10.1631/jzus.2005.B0981
Abstract( 2599 )     PDF(0KB)( 944 )
This work analyses the potential role of nitrogen pollution technology of crop systems of Pujiang, County in Eastern China’s Zhejiang Province, rice and vegetables are important cropping systems. We used a case study approach involving comparison of farmer practices and improved technologies. This approach allows assessing the impact of technology on pollution, is forward looking, and can yield information on the potential of on-the-shelf technology and provide opportunities for technology development. The approach particularly suits newly developed rice technologies with large potential of reducing nitrogen pollution and for future rice and vegetables technologies. The results showed that substantial reductions in nitrogen pollution are feasible for both types of crops.
Anatomical studies on water hyacinth (Eichhornia crassipes (Mart.) Solms) under the influence of textile wastewater
MAHMOOD Qaisar, ZHENG Ping, SIDDIQI M. Rehan, ISLAM Ejaz ul, AZIM M. Rashid, HAYAT Yousaf
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(10): 6-.   https://doi.org/10.1631/jzus.2005.B0991
Abstract( 2801 )     PDF(0KB)( 1459 )
Water hyacinth (Eichhornia crassipes (Mart.) Solms) is a prolific free floating aquatic macrohpyte found in tropical and subtropical parts of the earth. The effects of pollutants from textile wastewater on the anatomy of the plant were studied. Water hyacinth exhibits hydrophytic adaptations which include reduced epidermis cells lacking cuticle in most cases, presence of large air spaces (7~50 µm), reduced vascular tissue and absorbing structures. Textile waste significantly affected the size of root cells. The presence of raphide crystals was noted in parenchyma cells of various organs in treated plants.
Experimental study on the inhibition of biological reduction of Fe(III)EDTA in NOx absorption solution
LI Wei, WU Cheng-zhi, ZHANG Shi-han, SHI Yao, LEI Le-cheng
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(10): 8-.   https://doi.org/10.1631/jzus.2005.B1005
Abstract( 2144 )     PDF(0KB)( 1005 )
Scrubbing of NOx from the gas phase with Fe(II)EDTA has been shown to be highly effective. A new biological method can be used to convert NO to N2 and regenerate the chelating agent Fe(II)EDTA for continuous NO absorption. The core of this biological regeneration is how to effectively simultaneous reduce Fe(III)EDTA and Fe(II)EDTA-NO, two mainly products in the ferrous chelate absorption solution. The biological reduction rate of Fe(III)EDTA plays a main role for the NOx removal efficiency. In this paper, a bacterial strain identified as Klebsiella Trevisan sp. was used to demonstrate an inhibition of Fe(III)EDTA reduction in the presence of Fe(II)EDTA-NO. The competitive inhibition experiments indicted that Fe(II)EDTA-NO inhibited not only the growth rate of the iron-reduction bacterial strain but also the Fe(III)EDTA reduction rate. Cell growth rate and Fe(III)EDTA reduction rate decreased with increasing Fe(II)EDTA-NO concentration in the solution.
Strong influences of polymerization temperature on ethylene/1-hexene copolymerization catalyzed by (2-PhInd)2ZrCl2/methyl aluminoxane
YE Jian-ding, FAN Zhi-qiang, WANG Wei
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(10): 9-.   https://doi.org/10.1631/jzus.2005.B1009
Abstract( 2216 )     PDF(0KB)( 925 )
Ethylene/1-hexene was copolymerized by an unbridged zirconocene, (2-PhInd)2ZrCl2/MAO (methyl aluminoxane) at 0 °C and 50 °C respectively. High copolymerization activity and 1-hexene incorporation were observed at 0 °C, with the copolymer formed having random sequence distribution and narrow molecular weight distribution. Ethylene polymerization at 50 °C showed high activity, but copolymerization at 50 °C showed much lower activity, which decreased sharply with increasing 1-hexene concentration in the monomer feed. Copolymer formed at 50 °C showed blocky sequence distribution and broad molecular weight distribution. A mechanism model based on ligand rotation hindered by the propagation chain has been proposed to qualitatively explain the observed phenomena.
Investigation on the origin of 5-HMF in Shengmaiyin decoction by RP-HPLC method
LI Ying-hua, LV Xiu-yang
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(10): 10-.   https://doi.org/10.1631/jzus.2005.B1015
Abstract( 2970 )     PDF(0KB)( 1030 )
The origin of 5-HMF (5-hydroxymethyl-2-furaldehyde) in a Shengmaiyin decoction was investigated by the RP-HPLC method below. A C18 column (250 mm×4.6 mm, i.d. 5 μm) with a column temperature of 25 °C was used. The mobile phase was a mixture of ultra-pure water-acetonitrile (95:5, V/V) and the flow rate was 1.0 ml/min. The detection wavelength was 280 nm. The injection volume was 1 μl and the running time was about 20 min. The addition of Schisandra was regulated to assess the contribution of an acid environment to the production of 5-HMF. In order to confirm the role of saccharides in the production of 5-HMF, different amount of fructose was used. The 5-HMF level in decoctions of processed and unprocessed Schisandra was investigated in order to determine the origin of 5-HMF. The results showed that 5-HMF was derived mainly from the decoction of Schisandra only and not the mixed decoction of Ophionpogon and Schisandra. The appearance of 5-HMF is not simply the result of the decomposition of saccharides under the acid environment created by Schisandra, but the processing procedure plays an important role in the production of 5-HMF.
Removal of hexavalent chromium from aqueous solution by iron nanoparticles
NIU Shao-feng, LIU Yong, XU Xin-hua, LOU Zhang-hua
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(10): 11-.   https://doi.org/10.1631/jzus.2005.B1022
Abstract( 3415 )     PDF(0KB)( 1280 )
Groundwater remediation by nanoparticles has received increasing interest in recent years. This report presents a thorough evaluation of hexavalent chromium removal in aqueous solutions using iron (Fe0) nanoparticles. Cr(VI) is a major pollutant of groundwater. Zero-valent iron, an important natural reductant of Cr(VI), is an option in the remediation of contaminated sites, transforming Cr(VI) to essentially nontoxic Cr(III). At a dose of 0.4 g/L, 100% of Cr(VI) (20 mg/L) was degraded. The Cr(VI) removal efficiency decreased significantly with increasing initial pH. Different Fe0 type was compared in the same conditions. The reactivity was in the order starch-stabilized Fe0 nanoparticles>Fe0 nanoparticles>Fe0 powder>Fe0 filings. Electrochemical analysis of the reaction process led to the conclusion that Cr(OH)3 should be the final product of Cr(VI). Iron nanoparticles are good choice for the remediation of heavy metals in groundwater.
Influence of kaolinite on chiral hydrolysis of methyl dichlorprop enantiomers
FANG Zhao-hua, WEN Yue-zhong, LIU Wei-ping
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(10): 12-.   https://doi.org/10.1631/jzus.2005.B1028
Abstract( 2361 )     PDF(0KB)( 943 )
The effect of kaolinite on the enzymatic chiral hydrolysis of methyl dichlorprop enantiomers ((R,S)-methyl-2-(2,4- dichlorophenoxy) propanoic acid, 2,4-DPM) was investigated using chiral gas chromatography. Compared with the control without kaolinite, the enantiomeric ratio (ER) increased from 1.35 to 8.33 and the residual ratio of 2,4-DPM decreased from 60.89% to 41.55% in the presence of kaolinite. Kaolinite likely had emotion influence on lipase activity and its enantioselectivity. Moreover, the amount of kaolinite added was also found to be a sensitive factor affecting the enantioselective hydrolysis of 2,4-DPM. Fourier transform infrared (FTIR) spectroscopy studies of the interaction of lipase with kaolinite provided insight into the molecular structure of the complex and offered explanation of the effects of kaolinite on enzymatic hydrolysis of 2,4-DPM. Spectra showed that the effect of kaolinite on the hydrolysis of 2,4-DPM was affected by adsorption of lipase on kaolinite and changes of adsorbed lipase conformation, which led to the modified enantioselectivity.
Flocculation control study based on fractal theory
CHANG Ying, LIU Qian-jun, ZHANG Jin-song
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(10): 14-.   https://doi.org/10.1631/jzus.2005.B1038
Abstract( 2681 )     PDF(0KB)( 1354 )
A study on flocculation control based on fractal theory was carried out. Optimization test of chemical coagulant dosage confirmed that the fractal dimension could reflect the flocculation degree and settling characteristics of aggregates and the good correlation with the turbidity of settled effluent. So that the fractal dimension can be used as the major parameter for flocculation system control and achieve self-acting adjustment of chemical coagulant dosage. The fractal dimension flocculation control system was used for further study carried out on the effects of various flocculation parameters, among which are the dependency relationship among aggregates fractal dimension, chemical coagulant dosage, and turbidity of settled effluent under the conditions of variable water quality and quantity. And basic experimental data were obtained for establishing the chemical coagulant dosage control model mainly based on aggregates fractal dimension.
Biotechnology & Biomedicine
Optimization of conditions for supercritical fluid extraction of flavonoids from hops (Humulus lupulus L.)
HE Guo-qing, XIONG Hao-ping, CHEN Qi-he, RUAN Hui, WANG Zhao-yue, TRAORÉ Lonseny
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(10): 999-1004.   https://doi.org/10.1631/jzus.2005.B0999
Abstract( 2929 )     PDF(0KB)( 1234 )
Waste hops are good sources of flavonoids. Extraction of flavonoids from waste hops (SC-CO2 extracted hops) using supercritical fluids technology was investigated. Various temperatures, pressures and concentrations of ethanol (modifier) and the ratio (w/w) of solvent to material were tested in this study. The results of single factor and orthogonal experiments showed that at 50 °C, 25 MPa, the ratio of solvent to material (50%), ethanol concentration (80%) resulted in maximum extraction yield flavonoids (7.8 mg/g). HPLC-MS analysis of the extracts indicated that flavonoids obtained were xanthohumol, the principal prenylflavonoid in hops.
Chemical & Environmental Engineering
Isobaric vapor-liquid equilibrium for methyldichlorosilane-dimethyldichlorosilane-benzene system
QIU Zu-min, XIE Xin-liang, YU Shu-xian, CHEN Wen-you, XIE Feng-xia, LIU Juan
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(10): 1033-1037.   https://doi.org/10.1631/jzus.2005.B1033
Abstract( 2779 )     PDF(0KB)( 1243 )
The elucidation of vapor-liquid equilibrium (VLE) of the halogenated silane was necessary for the production of silicon derivatives, especially for methylvinyldichlorosilane, due to the lack of the relevant reports. Isobaric VLE for the system methyldichlorosilane-dimethyldichlorosilane-benzene and isobaric VLE of the three binary systems were measured with a new pump-ebulliometer at the pressure of 101.325 kPa. These binary compositions of the equilibrium vapor were calculated according to the Q function of molar excess Gibbs energy by the indirect method and the resulted VLE data agreed well with the thermodynamic consistency. Moreover, the experimental data were correlated with the Wilson, NRTL, Margules and van Laar equations by means of the least-squares fit, the acquired optimal interaction parameters were fitted to experimental vapor-liquid equilibrium data for binary systems. The binary parameters of Wilson equation were also used to calculate the bubble point temperature and the vapor phase composition for the ternary mixtures without any additional adjustment. The predicted vapor-liquid equilibrium for the ternary system was in a good agreement with the experimental results. The VLE of binary and multilateral systems provided essential theory for the production of the halogenated silane.
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