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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2016, Vol. 17 Issue (8): 610-618    DOI: 10.1631/jzus.B1500214
Articles     
Chryseobacterium chengduensis sp. nov. isolated from the air of captive giant panda enclosures in Chengdu, China
Cai-fang Wen, Li-xin Xi, Shan Zhao, Zhong-xiang Hao, Lu Luo, Hong Liao, Zhen-rong Chen, Rong She, Guo-quan Han, San-jie Cao, Rui Wu, Qi-gui Yan, Rong Hou
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; College of Food Science, Sichuan Agricultural University, Yaan 625014, China; Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China
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Abstract  A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1T, was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1T grew optimally at pH 7.0–8.0, at 28–30 °C and in the presence of NaCl concentrations from 0.0% to 0.5 %. 16S rRNA gene sequence analysis indicated that strain 25-1T belongs to the genus Chryseobacterium within the family Flavobacteriaceae and is related most closely to C. carnis G81T (96.4% similarity), C. lathyri RBA2-6T (95.8% similarity), and C. zeae JM1085T (95.8% similarity). Its genomic DNA G+C molar composition was 36.2%. The major cellular fatty acids were iso-C15:0 (44.0%), iso-C17:0 3OH (19.8%) and C16:1 ω7c/16:1 ω6c (12.7%). The only isoprenoid quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA–DNA relatedness between strain 25-1T and C. lathyri RBA2-6T was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1T is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1T (CCTCC AB2015133T=DSM 100396T).

Key words： Chryseobacterium chengduensis      Giant panda      16S rRNA sequencing      Phylogenetic analysis      Strain 25-1T     
Received: 05 September 2015      Published: 04 August 2016
CLC:  S865  
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Cai-fang Wen
Guo-quan Han
San-jie Cao
Rui Wu
Qi-gui Yan
Rong Hou
Li-xin Xi
Shan Zhao
Zhong-xiang Hao
Lu Luo
Hong Liao
Zhen-rong Chen
Rong She
Cite this article:

Cai-fang Wen, Li-xin Xi, Shan Zhao, Zhong-xiang Hao, Lu Luo, Hong Liao, Zhen-rong Chen, Rong She, Guo-quan Han, San-jie Cao, Rui Wu, Qi-gui Yan, Rong Hou. Chryseobacterium chengduensis sp. nov. isolated from the air of captive giant panda enclosures in Chengdu, China. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2016, 17(8): 610-618.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1500214     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2016/V17/I8/610

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