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| Molecularly imprinted polymers as the extracted sorbents of clenbuterol ahead of liquid chromatographic determination |
| Sovichea Lay, Hai-ning Yu, Bao-xiang Hu, Sheng-rong Shen |
| Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China; College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China; Department of Chemical Engineering and Material Science, Zhejiang University of Technology, Hangzhou 310032, China |
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Abstract A pre-treatment methodology for clenbuterol hydrochloride (CLEN) isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers (MIPs). CLEN-imprinted polymers were synthesized by the combined use of ally-β-cyclodextrin (ally-β-CD) and methacrylic acid (MAA), allyl-β-CD and acrylonitrile (AN), and allyl-β-CD and methyl methacrylate (MMA) as the binary functional monomers. MAA-linked allyl-β-CD MIPs (M-MAA) were characterized by Fourier transform-infrared (FT-IR) spectroscopy and a scanning electron microscope (SEM). Based upon the results, M-MAA polymers generally proved to be an excellent selective extraction compared to its references: AN-linked allyl-β-CD MIPs (M-AN) and MMA-linked allyl-β-CD MIPs (M-MMA). M-MAA polymers were eventually chosen to run through a molecularly imprinted solid-phase extraction (MISPE) micro-column to enrich CLEN residues spiked in pig livers. A high recovery was achieved, ranging from 91.03% to 96.76% with relative standard deviation (RSD) ≤4.45%.
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Received: 16 September 2015
Published: 03 June 2016
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