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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2015, Vol. 16 Issue (10): 845-856    DOI: 10.1631/jzus.B1400319
Articles     
Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit
Ning Zhang, Jing Jiang, Yan-li Yang, Zhi-he Wang
Key Laboratory of Protected Horticulture, Ministry of Education, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
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Abstract  In this study, we produced tomato plants overexpressing an invertase inhibitor gene (Sly-INH) from tomato, using a simple and efficient transient transformation system. Compared with control plants, the expression of Sly-INH was highly upregulated in Sly-INH overexpressing plants, as indicated by real-time polymerase chain reaction (PCR). Physiological analysis revealed that Sly-INH inhibited the activity of cell wall invertase (CWIN), which increased sugar accumulation in tomato fruit. Furthermore, Sly-INH mediated sucrose metabolism by regulating CWIN activity. Our results suggest that invertase activity is potentially regulated by the Sly-INH inhibitor at the post-translational level, and they demonstrate that the transient transformation system is an effective method for determining the functions of genes in tomato.

Key words： Invertase inhibitor      Fruit development      Transient transformation system      Solanum lycopersicum      Overexpression     
Received: 19 November 2014      Published: 03 October 2015
CLC:  R394-3  
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Ning Zhang
Jing Jiang
Yan-li Yang
Zhi-he Wang
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Ning Zhang, Jing Jiang, Yan-li Yang, Zhi-he Wang. Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2015, 16(10): 845-856.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1400319     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2015/V16/I10/845

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