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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2015, Vol. 16 Issue (5): 395-403    DOI: 10.1631/jzus.B1400224
Articles     
Cisplatin combined with hyperthermia kills HepG2 cells in intraoperative blood salvage but preserves the function of erythrocytes
Jin-ting Yang, Li-hui Tang, Yun-qing Liu, Yin Wang, Lie-ju Wang, Feng-jiang Zhang, Min Yan
Department of Anesthesiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221004, China
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Abstract  The safe use of intraoperative blood salvage (IBS) in cancer surgery remains controversial. Here, we investigated the killing effect of cisplatin combined with hyperthermia on human hepatocarcinoma (HepG2) cells and erythrocytes from IBS in vitro. HepG2 cells were mixed with concentrated erythrocytes and pretreated with cisplatin (50, 100, and 200 μg/ml) alone at 37 °C for 60 min and cisplatin (25, 50, 100, and 200 μg/ml) combined with hyperthermia at 42 °C for 60 min. After pretreatment, the cell viability, colony formation and DNA metabolism in HepG2 and the Na+-K+-ATPase activity, 2,3-diphosphoglycerate (2,3-DPG) concentration, free hemoglobin (Hb) level, osmotic fragility, membrane phosphatidylserine externalization, and blood gas variables in erythrocytes were determined. Pretreatment with cisplatin (50, 100, and 200 μg/ml) combined with hyperthermia (42 °C) for 60 min significantly decreased HepG2 cell viability, and completely inhibited colony formation and DNA metabolism when the HepG2 cell concentration was 5×104 ml−1 in the erythrocyte (P<0.01). Erythrocytic Na+-K+-ATPase activity, 2,3-DPG level, phosphatidylserine externalization, and extra-erythrocytic free Hb were significantly altered by hyperthermia plus high concentrations of cisplatin (100 and 200 μg/ml) (P<0.05), but not by hyperthermia plus 50 μg/ml cisplatin (P>0.05). In conclusion, pretreatment with cisplatin (50 μg/ml) combined with hyperthermia (42 °C) for 60 min effectively eliminated HepG2 cells from IBS but did not significantly affect erythrocytes in vitro.

Key words： Erythrocytes      HepG2 cells      Intraoperative blood salvage      Cisplatin      Hyperthermia     
Received: 11 August 2014      Published: 04 May 2015
CLC:  R735  
  R457  
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Jin-ting Yang
Li-hui Tang
Yun-qing Liu
Yin Wang
Lie-ju Wang
Feng-jiang Zhang
Min Yan
Cite this article:

Jin-ting Yang, Li-hui Tang, Yun-qing Liu, Yin Wang, Lie-ju Wang, Feng-jiang Zhang, Min Yan. Cisplatin combined with hyperthermia kills HepG2 cells in intraoperative blood salvage but preserves the function of erythrocytes. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2015, 16(5): 395-403.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1400224     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2015/V16/I5/395

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