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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2014, Vol. 15 Issue (5): 482-490    DOI: 10.1631/jzus.B1300203
Articles     
Adipose-derived stem cells transfected with pEGFP-OSX enhance bone formation during distraction osteogenesis
Qing-guo Lai, Shao-long Sun, Xiao-hong Zhou, Chen-ping Zhang, Kui-feng Yuan, Zhong-jun Yang, Sheng-lei Luo, Xiao-peng Tang, Jiang-bo Ci
Department of Oral and Maxillofacial Surgery, the Second Hospital of Shandong University, Jinan 250033, China; Department of Oral and Maxillofacial Surgery, Shanghai Key Laboratory of Stomatology, the Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China; Department of Stomatology, Zhaoyuan People's Hospital, Zhaoyuan 265400, China; School of Stomatology, Shandong University, Jinan 250012, China; Department of Ultrasonography, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China
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Abstract  This study was designed to investigate the effects of local delivery of adipose-derived stem cells (ADSCs) transfected with transcription factor osterix (OSX) on bone formation during distraction osteogenesis. New Zealand white rabbits (n=54) were randomly divided into three groups (18 rabbits per group). A directed cloning technique was used for the construction of recombinant plasmid pEGFP-OSX, where EGFP is the enhanced green fluorescence protein. After osteodistraction of the right mandible of all experimental rabbits, rabbits in group A were treated with ADSCs transfected with pEGFP-OSX, group B with ADSCs transfected with pEGFP-N1, and group C with physiological saline. Radiographic and histological examinations were processed after half of the animals within each group were humanely killed by injection of sodium pentothal at Week 2 or 6 after surgery. The distraction bone density was measured as its projectional bone mineral density (BMD). Three parameters were measured, namely, the thickness of new trabeculae (TNT), and the volumes of the newly generated cortical bone (NBV1) and the cancellous bone (NBV2) of the distracted regions. Good bone generation in the distraction areas was found in group A, which had the highest BMD, TNT, and NBV in the distraction zones among the groups. There was no significant difference in bone generation in the distraction areas between groups B and C. The results indicate that the transplantation of ADSCs transfected with pEGFP-OSX can effectively promote bone generation during distraction in vivo.

Key words： Adipose-derived stem cell (ADSC)      Distraction osteogenesis      Transcription factor      Gene transfection     
Received: 01 August 2013      Published: 05 May 2014
CLC:  R782.2  
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Jiang-bo Ci
Qing-guo Lai
Chen-ping Zhang
Shao-long Sun
Xiao-hong Zhou
Kui-feng Yuan
Zhong-jun Yang
Sheng-lei Luo
Xiao-peng Tang
Cite this article:

Qing-guo Lai, Shao-long Sun, Xiao-hong Zhou, Chen-ping Zhang, Kui-feng Yuan, Zhong-jun Yang, Sheng-lei Luo, Xiao-peng Tang, Jiang-bo Ci. Adipose-derived stem cells transfected with pEGFP-OSX enhance bone formation during distraction osteogenesis. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2014, 15(5): 482-490.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1300203     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2014/V15/I5/482

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