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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2014, Vol. 15 Issue (3): 289-294    DOI: 10.1631/jzus.B1300083
Articles     
Effect of vitamin B12 on cleft palate induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and dexamethasone in mice
Shu-fan Zhao, Mao-zhou Chai, Min Wu, Yong-hong He, Tian Meng, Bing Shi
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Cleft Lip and Palate Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Biochemistry & Molecular Biology, University of North Dakota School of Medicine & Health Sciences, Grand Forks, ND 58203, USA
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Abstract  The purpose of this study was to investigate the effect of vitamin B12 on palatal development by co-administration of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and dexamethasone (DEX). We examined the morphological and histological features of the palatal shelf and expression levels of key signaling molecules (transforming growth factor-β3 (TGF-β3) and TGF-β type I receptor (activin receptor-like kinase 5, ALK5)) during palatogenesis among a control group (Group A), TCDD+DEX exposed group (Group B), and TCDD+DEX+vitamin B12 exposed group (Group C). While we failed to find that vitamin B12 decreased the incidence of cleft palate induced by TCDD+DEX treatment, the expression levels of key signaling molecules (TGF-β3 and ALK5) during palatogenesis were significantly modulated. In TCDD+DEX exposed and TCDD+DEX+vitamin B12 exposed groups, palatal shelves could not contact in the midline due to their small sizes. Our results suggest that vitamin B12 may inhibit the expression of some cleft palate inducers such as TGF-β3 and ALK5 in DEX+TCDD exposed mice, which may be beneficial against palatogenesis to some degree, even though we were unable to observe a protective role of vitamin B12 in morphological and histological alterations of palatal shelves induced by DEX and TCDD.

Key words： Cleft palate      Transforming growth factor-β3 (TGF-β3)      Activin receptor-like kinase 5 (ALK5)      Vitamin B12      8-Tetrachlorodibenzo-p-dioxin     
Received: 22 March 2013      Published: 04 March 2014
CLC:  R780.1  
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Shu-fan Zhao
Yong-hong He
Mao-zhou Chai
Min Wu
Tian Meng
Bing Shi
Cite this article:

Shu-fan Zhao, Mao-zhou Chai, Min Wu, Yong-hong He, Tian Meng, Bing Shi. Effect of vitamin B12 on cleft palate induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and dexamethasone in mice. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2014, 15(3): 289-294.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1300083     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2014/V15/I3/289

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