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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2013, Vol. 14 Issue (7): 615-620    DOI: 10.1631/jzus.B1200361
    
Separation of mandelic acid and its derivatives with new immobilized cellulose chiral stationary phase
Jie Zhou, Qian Liu, Guang-jun Fu, Zhen-zhong Zhang
Key Laboratory of State Ministry of Education for Pharmaceutical Technology, School of Pharmacy, Zhengzhou University, Zhengzhou 450001, China
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Abstract  A new liquid chromatographic method has been developed for the chiral separation of the enantiomers of mandelic acid and their derivatives 2-chloromandelic acid, 4-hydroxymandelic acid, 4-methoxymandelic acid, and 3,4,5-trismethoxymandelic acid. The enantiomers were separated by a CHIRALPAK® IC (250 mm×4.6 mm, 5 μm). Mandelic acid, 4-methoxymandelic acid, and 3,4,5-trismethoxymandelic acid were baseline resolved (resolution factor (RS)=2.21, RS=2.14, and RS=3.70, respectively). In contrast, the enantioselectivities between CHIRALPAK® IC and 2-chloromandelic acid and 4-hydroxymandelic acid investigated were low. By comparing the chromatographs of mandelic acid enantiomers and mandelic acid spiked with (R)-mandelic acid, it was determined that the first effluent was (R)-mandelic acid.

Key words： CHIRALPAK® IC      Enantiomeric separation      High-performance liquid chromatography (HPLC)      Mandelic acid      Derivatives     
Received: 27 December 2012      Published: 04 July 2013
CLC:  R917  
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Jie Zhou
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Zhen-zhong Zhang
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Jie Zhou, Qian Liu, Guang-jun Fu, Zhen-zhong Zhang. Separation of mandelic acid and its derivatives with new immobilized cellulose chiral stationary phase. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2013, 14(7): 615-620.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1200361     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2013/V14/I7/615

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