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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2011, Vol. 12 Issue (11): 935-942    DOI: 10.1631/jzus.B1000417
    
DNA methylation polymorphism in flue-cured tobacco and candidate markers for tobacco mosaic virus resistance
Jie-hong Zhao, Ji-shun Zhang, Yi Wang, Ren-gang Wang, Chun Wu, Long-jiang Fan, Xue-liang Ren
Guizhou Institute of Tobacco Science, Guiyang 550081, China, Department of Agronomy, Zhejiang University, Hangzhou 310029, China
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Abstract  DNA methylation plays an important role in the epigenetic regulation of gene expression during plant growth, development, and polyploidization. However, there is still no distinct evidence in tobacco regarding the distribution of the methylation pattern and whether it contributes to qualitative characteristics. We studied the levels and patterns of methylation polymorphism at CCGG sites in 48 accessions of allotetraploid flue-cured tobacco, Nicotiana tabacum, using a methylation-sensitive amplified polymorphism (MSAP) technique. The results showed that methylation existed at a high level among tobacco accessions, among which 49.3% sites were methylated and 69.9% allelic sites were polymorphic. A cluster analysis revealed distinct patterns of geography-specific groups. In addition, three polymorphic sites significantly related to tobacco mosaic virus (TMV) resistance were explored. This suggests that tobacco breeders should pay more attention to epigenetic traits.

Key words： Methylation-sensitive amplified polymorphism      Epigenetic modification      Tobacco mosaic virus (TMV) resistance      Flue-cured tobacco     
Received: 03 December 2010      Published: 04 November 2011
CLC:  Q341  
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Jie-hong Zhao
Ji-shun Zhang
Yi Wang
Ren-gang Wang
Chun Wu
Long-jiang Fan
Xue-liang Ren
Cite this article:

Jie-hong Zhao, Ji-shun Zhang, Yi Wang, Ren-gang Wang, Chun Wu, Long-jiang Fan, Xue-liang Ren. DNA methylation polymorphism in flue-cured tobacco and candidate markers for tobacco mosaic virus resistance. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2011, 12(11): 935-942.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1000417     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2011/V12/I11/935

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