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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2010, Vol. 11 Issue (1): 10-16    DOI: 10.1631/jzus.B0900163
Biomedicine     
Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing
Tie-lian LIU, Jing-cheng MIAO, Wei-hua SHENG, Yu-feng XIE, Quan HUANG, Yun-bo SHAN
Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China; Laboratory Department of the Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China; Department of Renal Medicine, Nantong Municipal No. 1 People\'s Hospital, Nantong 226001, China
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Abstract  Objective: To explore the feasibility of using regenerated silk fibroin membrane to construct artificial skin substitutes for wound healing, it is necessary to evaluate its cytocompatibility. Methods: The effects of regenerated silk fibroin film on cytotoxicity, adhesion, cell cycle, and apoptosis of L929 cells, growth and vascular endothelial growth factor (VEGF) expression of ECV304 cells, and VEGF, angiopoietin-1 (Ang-1), platelet-derived growth factor (PDGF) and fibroblast growth factor 2 (FGF2) expression of WI-38 cells were assessed by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay, viable cell counting, flow cytometry (FCM), and enzyme-linked immunosorbant assay (ELISA). Results: We showed that the regenerated silk fibroin film was not cytotoxic to L929 cells and had no adverse influence on their adhesion, cell cycle or apoptosis; it had no adverse influence on the growth and VEGF secretion of ECV304 cells and no effect on the secretion of VEGF, Ang-1, PDGF and FGF2 by WI-38 cells. Conclusion: The regenerated silk fibroin film should be an excellent biomaterial with good cytocompatibility, providing a framework for reparation after trauma in clinical applications.

Key words： Regenerated silk fibroin film      Cytocompatibility      Cytotoxicity     
Received: 06 September 2009      Published: 01 January 2010
CLC:  R64  
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Tie-lian LIU
Jing-cheng MIAO
Wei-hua SHENG
Yu-feng XIE
Quan HUANG
Yun-bo SHAN
Cite this article:

Tie-lian LIU, Jing-cheng MIAO, Wei-hua SHENG, Yu-feng XIE, Quan HUANG, Yun-bo SHAN. Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(1): 10-16.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0900163     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2010/V11/I1/10

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