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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2009, Vol. 10 Issue (6): 411-421    DOI: 10.1631/jzus.B0820400
Biotechnology     
Epigallocatechin-3-gallate affects the growth of LNCaP cells via membrane fluidity and distribution of cellular zinc
Jun-guo YANG, Hai-ning YU, Shi-li SUN, Lan-cui ZHANG, Guo-qing HE, Undurti N. DAS, Hui RUAN, Sheng-rong SHEN
Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China; College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310032, China; Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; UND Life Sciences, 13800 Fairhill Road, #321, Shaker Heights, OH 44120, USA
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Abstract  Objective: To evaluate effects of epigallocatechin-3-gallate (EGCG) on the viability, membrane properties, and zinc distribution, with and without the presence of Zn2+, in human prostate carcinoma LNCaP cells. Methods: We examined changes in cellular morphology and membrane fluidity of LNCaP cells, distribution of cellular zinc, and the incorporated portion of EGCG after treatments with EGCG, Zn2+, and EGCG+Zn2+. Results: We observed an alteration in cellular morphology and a decrease in membrane fluidity of LNCaP cells after treatment with EGCG or Zn2+. The proportion of EGCG incorporated into liposomes treated with the mixture of EGCG and Zn2+ at the ratio of 1:1 was 90.57%, which was significantly higher than that treated with EGCG alone (30.33%). Electron spin resonance (ESR) studies and determination of fatty acids showed that the effects of EGCG on the membrane fluidity of LNCaP were decreased by Zn2+. EGCG accelerated the accumulation of zinc in the mitochondria and cytosol as observed by atomic absorption spectrometer. Conclusion: These results show that EGCG interacted with cell membrane, decreased the membrane fluidity of LNCaP cells, and accelerated zinc accumulation in the mitochondria and cytosol, which could be the mechanism by which EGCG inhibits proliferation of LNCaP cells. In addition, high concentrations of Zn2+ could attenuate the actions elicited by EGCG.

Key words： Epigallocatechin-3-gallate (EGCG)      Zinc      LNCaP      Membrane fluidity     
Received: 20 December 2008     
CLC:  Q2  
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Jun-guo YANG
Hai-ning YU
Shi-li SUN
Lan-cui ZHANG
Guo-qing HE
Undurti N. DAS
Hui RUAN
Sheng-rong SHEN
Cite this article:

Jun-guo YANG, Hai-ning YU, Shi-li SUN, Lan-cui ZHANG, Guo-qing HE, Undurti N. DAS, Hui RUAN, Sheng-rong SHEN. Epigallocatechin-3-gallate affects the growth of LNCaP cells via membrane fluidity and distribution of cellular zinc. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2009, 10(6): 411-421.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0820400     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2009/V10/I6/411

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