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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2007, Vol. 8 Issue (7): 458-464    DOI: 10.1631/jzus.2007.B0458
Biotechnology     
Effects of osmotic stress on antioxidant enzymes activities in leaf discs of PSAG12-IPT modified gerbera
LAI Qi-xian, BAO Zhi-yi, ZHU Zhu-jun, QIAN Qiong-qiu, MAO Bi-zeng
Key Laboratory of Horticultural Plant Development and Biotechnology, Department of Horticulture, Zhejiang University, Hangzhou 310029, China; Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China; Department of Horticulture, Zhejiang Forestry University, Lin’an 311300, China
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Abstract  Leaf senescence is often caused by water deficit and the chimeric gene PSAG12-IPT is an auto-regulated gene delaying leaf senescence. Using in vitro leaf discs culture system, the changes of contents of chlorophylls, carotenoids, soluble protein and thiobarbituric acid reactive substance (TBARS) and antioxidant enzymes activities were investigated during leaf senescence of PSAGl2-IPT modified gerbera induced by osmotic stress compared with the control plant (wild type). Leaf discs were incubated in 20%, 40% (w/v) polyethylene glycol (PEG) 6 000 nutrient solution for 20 h under continuous light [130 µmol/(m2·s)]. The results showed that the contents of chlorophylls, carotenoids and soluble protein were decreased by osmotic stress with the decrease being more pronounced at 40% PEG, but that, at the same PEG concentration the decrease in the transgenic plants was significantly lower than that in the control plant. The activities of superoxide dismutase (SOD), catalases (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and dehydroascorbate reductase (DHAR) were stimulated by PEG treatment. However, the increases were higher in PSAG12-IPT transgenic plants than in the control plants, particularly at 40% PEG treatment. Lipid peroxidation (TBARS content) was increased by PEG treatment with the increase being much lower in transgenic plant than in the control plant. It could be concluded that the increases in the activities of antioxidant enzymes including SOD, CAT, APX, GPX and DHAR were responsible for the delay of leaf senescence induced by osmotic stress.

Key words： Antioxidant enzymes      Gerbera      Leaf disc      Leaf senescence      Osmotic stress      PSAG12-IPT     
Received: 26 September 2006     
CLC:  S601  
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LAI Qi-xian
BAO Zhi-yi
ZHU Zhu-jun
QIAN Qiong-qiu
MAO Bi-zeng
Cite this article:

LAI Qi-xian, BAO Zhi-yi, ZHU Zhu-jun, QIAN Qiong-qiu, MAO Bi-zeng. Effects of osmotic stress on antioxidant enzymes activities in leaf discs of PSAG12-IPT modified gerbera. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2007, 8(7): 458-464.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2007.B0458     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2007/V8/I7/458

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