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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2015, Vol. 16 Issue (11): 940-947    DOI: 10.1631/jzus.B1500055
Articles     
Morphological and physiological comparison of taxa comprising the Sporothrix schenckii complex
Ming-dan Zhao, Xun Zhou, Ting-ting Liu, Zhi-bang Yang
Department of Dermatology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Department of Dermatology and Cosmetology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, China; The Laboratory of Pathogen Biology and Immunology, Basic Medical Experiment Teaching Center, Chongqing Medical University, Chongqing 400016, China
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Abstract  Based on recent molecular data, it has been suggested that Sporothrix globosa is the main causal agent of sporotrichosis in China. The objective of this study was to compare the morphology, growth characteristics, patterns of carbon source usage, and susceptibility to antifungal agents among Sporothrix strains. A total of 15 clinical strains confirmed to be S. globosa, from three different regions of China, and 11 ex-type strains from the CBS-KNAW biodiversity center were obtained. The elongated conidia of S. pallida, S. variecibatus, S. schenckii, and S. schenckii luriei were clearly different from the subglobose and globose conidia of S. globosa strains. S. schenckii is able to assimilate sucrose, raffinose, and ribitol. Susceptibility profiles of these Sporothrix species were evaluated by measuring minimum inhibitory concentrations (MICs). Fluconazole, itraconazole, terbinafine, and amphotericin B showed good activity against most S. globosa clinical isolates from China. Potassium iodide also showed a low MIC against S. pallida, while fluconazole showed a high MIC for S. mexicana, S. humicola, S. globosa, S. schenckii, and S. inflata; these strains might be considered tolerant. The species showed differences in susceptibility to antifungal drugs and should therefore be properly identified during diagnosis prior to designing therapeutic strategies.

Key words： Sporothrix globosa      Phenotypic characters      Antifungal susceptibility     
Received: 08 March 2015      Published: 04 November 2015
CLC:  R375  
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Ming-dan Zhao
Xun Zhou
Ting-ting Liu
Zhi-bang Yang
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Ming-dan Zhao, Xun Zhou, Ting-ting Liu, Zhi-bang Yang. Morphological and physiological comparison of taxa comprising the Sporothrix schenckii complex. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2015, 16(11): 940-947.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1500055     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2015/V16/I11/940

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