Please wait a minute...
ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2012, Vol. 13 Issue (12): 1006-1014    DOI: 10.1631/jzus.B1200089
Articles     
An Arabidopsis mutant atcsr-2 exhibits high cadmium stress sensitivity involved in the restriction of H2S emission
Ya-wei Li, Ze-hua Gong, Yao Mu, Yi-xian Zhang, Zeng-jie Qiao, Li-ping Zhang, Zhu-ping Jin, Hua Li, Yan-xi Pei
School of Life Science, Shanxi University, Taiyuan 030006, China; School of Chemical Engineering and Environment, North University of China, Taiyuan 030051, China; School of Environmental Science and Resources, Shanxi University, Taiyuan 030006, China
Download:     PDF (0 KB)     
Export: BibTeX | EndNote (RIS)      

Abstract  The gene AtCSR encodes peptidyl-prolyl cis/trans isomerases (PPIases) that accelerate energetically unfavorable cis/trans isomerization of the peptide bond preceding proline production. In our studies, we found that AtCSR was associated with cadmium (Cd)-sensitive response in Arabidopsis. Our results show that AtCSR expression was triggered by Cd-stress in wild type Arabidopsis. The expression of some genes responsible for Cd2+ transportation into vacuoles was induced, and the expression of the iron-regulated transporter 1 (IRT1) related to Cd2+ absorption from the environment was not induced in wild type with Cd2+ treatment. The expression of Cd-transportation related genes was not in response to Cd-stress, whereas IRT expression increased dramatically in atcsr-2 with Cd2+ treatment. The expression of glutathione 1 (GSH1) was consistent with GSH being much lower in atcsr-2 in comparison with the wild type with Cd2+ treatment. Additionally, malondialdehyde (MDA), hydrogen peroxide, and Cd2+ contents, and activities of some antioxidative enzymes, differed between the wild type and atcsr-2. Hydrogen sulfide (H2S) has been confirmed as the third gas-transmitter over recent years. The findings revealed that the expression pattern of H2S-releasing related genes and that of Cd-induced chelation and transportation genes matched well in the wild type and atcsr-2, and H2S could regulate the expression of the Cd-induced genes and alleviate Cd-triggered toxicity. Finally, one possible suggestion was given: down-regulation of atcsr-2, depending on H2S gas-transmitter not only weakened Cd2+ chelation, but also reduced Cd2+ transportation into vacuoles, as well as enhancing the Cd2+ assimilation, thus rendering atcsr-2 mutant sensitive to Cd-stress.

Key words： AtCSR      H2S gas-transmitter      Glutathione      Cadmium stress sensitivity     
Received: 21 March 2012      Published: 07 December 2012
CLC:  Q945.78  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
Ya-wei Li
Yan-xi Pei
Ze-hua Gong
Yao Mu
Yi-xian Zhang
Zeng-jie Qiao
Li-ping Zhang
Zhu-ping Jin
Hua Li
Cite this article:

Ya-wei Li, Ze-hua Gong, Yao Mu, Yi-xian Zhang, Zeng-jie Qiao, Li-ping Zhang, Zhu-ping Jin, Hua Li, Yan-xi Pei. An Arabidopsis mutant atcsr-2 exhibits high cadmium stress sensitivity involved in the restriction of H2S emission. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2012, 13(12): 1006-1014.

URL:

http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1200089     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2012/V13/I12/1006

[1] Yue-en CHAO, Min ZHANG, Sheng-ke TIAN, Ling-li LU, Xiao-e YANG. Differential generation of hydrogen peroxide upon exposure to zinc and cadmium in the hyperaccumulating plant specie (Sedum alfredii Hance)[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2008, 9(3): 243-249.
[2] LI Hui-chun, CHEN Qiao-zhen, MA Ying, ZHOU Jun-fu. Imbalanced free radicals and antioxidant defense systems in schizophrenia: A comparative study[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(12): 7-.
[3] THYAGARAJU K., HEMAVATHI B., VASUNDHARA K., RAO A.D., DEVI K.N.. Comparative study on glutathione transferases of rat brain and testis under the stress of phenobarbitol and β-methylcholanthrene[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(8): 759-769.