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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2012, Vol. 13 Issue (1): 1-10    DOI: 10.1631/jzus.B1100216
Articles     
Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation
Yue-hong Lu, Hui-juan Gao, Bai-jia Li, Ying-ming Zheng, Ying-hui Ye, Yu-li Qian, Chen-ming Xu, He-feng Huang, Fan Jin
Department of Reproductive Endocrinology, Key Laboratory of Reproductive Genetics, Ministry of Education, and Key Laboratory of Women's Reproductive Health of Zhejiang Province, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China, Department of Reproductive Health, Shaoxing Women and Children Hospital, Shaoxing 312000, China
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Abstract  To evaluate the effects of sperm with different parameters and sources on the outcomes of intracytoplasmic sperm injection (ICSI), 1972 ICSI cycles were analyzed retrospectively. Groups 1 to 5 were composed of cycles using ejaculated sperm and were grouped according to sperm quantity, quality, and morphology into normal (288 cycles), or mild (329 cycles), moderate (522 cycles), severe (332 cycles), and extremely severe (171 cycles) oligozoospermia and/or asthenozoospermia and/or teratozoospermia (OAT) groups. Group 6 was composed of 250 cycles using testicular or epididymal sperm, and Group 7 consisted of 80 cycles using frozen-thawed sperm. We found that fertilization rates were gradually reduced from Groups 1 to 6, and reached statistical difference in Groups 5 and 6 (P<0.05). The high-quality embryo rate was higher in Group 1 than in Groups 2, 3, 5, 6, and 7 (P<0.05). No statistical differences were observed in the rates of embryo cleavage, clinical pregnancy, miscarriage, live-birth, premature birth, low birth weight, weeks of premature birth, average birth weight, or sex ratio for all seven groups (P>0.05). A total of nine cases of malformation were observed, with a malformation rate of 1.25% (9/719). In conclusion, different sperm sources and parameters can affect ICSI outcomes before embryo implantation. A full assessment of offspring malformation will require further study using a larger sample size.

Key words： Intracytoplasmic sperm injection (ICSI)      Sperm      Sperm source      Sperm parameters      Malformation     
Received: 06 July 2011      Published: 30 December 2011
CLC:  R71  
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Yue-hong Lu
Fan Jin
Hui-juan Gao
Bai-jia Li
Ying-ming Zheng
Ying-hui Ye
Yu-li Qian
Chen-ming Xu
He-feng Huang
Cite this article:

Yue-hong Lu, Hui-juan Gao, Bai-jia Li, Ying-ming Zheng, Ying-hui Ye, Yu-li Qian, Chen-ming Xu, He-feng Huang, Fan Jin. Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2012, 13(1): 1-10.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1100216     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2012/V13/I1/1

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