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| Allograft rejection-related gene expression in the endothelial cells of renal transplantation recipients after cytomegalovirus infection |
| Yang LI, Hang YAN, Wu-jun XUE, Pu-xun TIAN, Xiao-ming DING, Xiao-ming PAN, Xin-shun FENG, Xiao-hui TIAN, He-li XIANG, Jun HOU |
| Department of Renal Transplantation, Center of Nephropathy, the First Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China |
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Abstract Objective: To explore the effects of cytomegalovirus (CMV) infection on rejection-related gene expression in the endothelial cells of renal transplantation recipients. Methods: Endothelial cells (ECs) were cultured and stimulated by a variety of factors: A, normal control group; B, inactivated human cytomegalovirus (HCMV) infection group; C, HCMV infection group; D, HCMV supernatant infection group; and E, ganciclovir HCMV group. Expression of intercellular adhesion molecule-1 (ICAM-1) and major histocompability complex (MHC) class I and class II antigens was detected by flow cytometry (FCM) and immunohistochemistry. Results: We found characteristic CMV-infected ECs in this study. There were no significant differences among groups A, B and D (P>0.05). Although the expression levels of ICAM-1 were not significantly different between groups C and E (P>0.05), the ICAM-1 expression in these two groups was significantly higher than that in group A (P<0.05). ICAM-1 expression was detected in groups C and E, while there was no expression in groups A, B and D. Furthermore, there was no significant difference of ICAM-1 mRNA expression between groups C and E (P>0.05). Human leucocyte antigen (HLA)-ABC expression was detected in all the groups, while HLA-DR expression was only detected in groups C and E. There were no significant differences of HLA-ABC and HLA-DR expression among groups A, B and D (P>0.05). However, the HLA-ABC and HLA-DR expression levels in groups C and D were higher than those of the remaining groups previously reported (P<0.05). Meanwhile, the HLA-ABC and HLA-DR expression levels in group E were lower than those of group C (P<0.05). Conclusion: CMV could up-regulate the expression levels of ICAM-1 and MHC antigens, which was closely related to allograft rejection.
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Received: 21 April 2009
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Cite this article:
Yang LI, Hang YAN, Wu-jun XUE, Pu-xun TIAN, Xiao-ming DING, Xiao-ming PAN, Xin-shun FENG, Xiao-hui TIAN, He-li XIANG, Jun HOU. Allograft rejection-related gene expression in the endothelial cells of renal transplantation recipients after cytomegalovirus infection. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2009, 10(11): 820-828.
URL:
http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0920115 OR http://www.zjujournals.com/xueshu/zjus-b/Y2009/V10/I11/820
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