| Biomedicine |
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| Solid lipid nanoparticles loading adefovir dipivoxil for antiviral therapy |
| Xing-guo ZHANG, Jing MIAO, Min-wei LI, Sai-ping JIANG, Fu-qiang HU, Yong-zhong DU |
| Department of Pharmacy, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China; State Key Lab for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310003, China |
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Abstract Herein, solid lipid nanoparticles (SLN) were proposed as a new drug delivery system for adefovir dipivoxil (ADV). The octadecylamine-fluorescein isothiocynate (ODA-FITC) was synthesized and used as a fluorescence maker to be incorporated into SLN to investigate the time-dependent cellular uptake of SLN by HepG2.2.15. The SLN of monostearin with ODA-FITC or ADV were prepared by solvent diffusion method in an aqueous system. About 15 wt% drug entrapment efficiency (EE) and 3 wt% drug loading (DL) could be reached in SLN loading ADV. Comparing with free ADV, the inhibitory effects of ADV loaded in SLN on hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg) and hepatitis B virus (HBV) DNA levels in vitro were significantly enhanced.
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Received: 16 February 2008
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