| Biomedicine |
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| Comparison in effect of different metal ions, pH and reducing agent on the protease activity in human hyper mature and mature cataract |
| SAMI Amtul Jamil, SAMI Amtul Naseer, KANWAL Noreen |
| Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore 54594, Pakistan; Institute of Ophthalmology, King Edward Medical University, Lahore 54594, Pakistan |
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Abstract This study was undertaken to isolate and characterize the protease activity of human eye lens sample of mature and hyper mature cataract. Samples were collected just after surgery of the cataract lens and were stored at −20 °C. The total protein extract was isolated from 5 samples in each case (mature and hyper mature cataract) and clear supernatant obtained after centrifugation was used as an enzyme source. The optimum pH for the proteases of mature cataract was 7.5 while the proteases of hyper mature cataract were recorded for maximum activity at pH 5.5 and 7.5. The optimum temperature for both enzyme sources was 50 °C. Effect of different metal ions such as potassium, lead, silver, zinc and borate was studied. In each case protease activity was increased. Reducing agent e.g. β mercaptoethanol also caused an increase in activity indicating the involvement of sulfhydryl groups. Protease activity was also located on agar plates.
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Received: 14 March 2007
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