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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2007, Vol. 8 Issue (1): 70-75    DOI: 10.1631/jzus.2007.B0070
Biomedicine     
Hypertonic saline resuscitation maintains a more balanced profile of T-lymphocyte subpopulations in a rat model of hemorrhagic shock
LU Yuan-qiang, CAI Xiu-jun, GU Lin-hui, MU Han-zhou, HUANG Wei-dong
Department of Emergency and General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China; Cancer Institute, Zhejiang Tumor Hospital, Hangzhou 310022, China
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Abstract  Objective: To investigate the potential and early effect of hypertonic saline resuscitation on T-lymphocyte subpopulations in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock was established in 18 Sprague-Dawley (SD) rats. The rats were randomly divided into Sham group, HTS group (hypertonic saline resuscitation group) and NS group (normal saline resuscitation group). Each group contained 6 rats. The CD4+ and CD8+ subpopulations of T-lymphocytes in peripheral blood were detected respectively before shock and after resuscitation by double antibody labelling and flow cytometry. Results: In the early stage after hemorrhagic shock, fluid resuscitation and emergency treatment, the CD4+ lymphocytes of peripheral blood in HTS and NS groups markedly increased. Small volume resuscitation with HTS also induced peripheral CD8+ lymphocytes to a certain extent, whereas NS resuscitation showed no effect in this respect. Consequently, compared with Sham and HTS groups, CD4+/CD8+ ratio of peripheral blood in NS group was obviously increased, and showed statistically differences. Conclusion: In this model of rat with severe hemorrhagic shock, small volume resuscitation with HTS is more effective than NS in reducing immunologic disorders and promoting a more balanced profile of T-lymphocyte subpopulations regulating network.

Key words： Hemorrhagic shock      Resuscitation      Sodium chloride solution      Hypertonic saline      T-lymphocyte subpopulations      Flow cytometry     
Received: 28 March 2006     
CLC:  R605.971  
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LU Yuan-qiang
CAI Xiu-jun
GU Lin-hui
MU Han-zhou
HUANG Wei-dong
Cite this article:

LU Yuan-qiang, CAI Xiu-jun, GU Lin-hui, MU Han-zhou, HUANG Wei-dong. Hypertonic saline resuscitation maintains a more balanced profile of T-lymphocyte subpopulations in a rat model of hemorrhagic shock. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2007, 8(1): 70-75.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2007.B0070     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2007/V8/I1/70

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