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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2006, Vol. 7 Issue (10): 6-    DOI: 10.1631/jzus.2006.B0817
    
Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum
ZHENG Yi-xiong, RINGE Jochen, LIANG Zhong, LOCH Alexander, CHEN Li, SITTINGER Michael
Department of Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; Tissue Engineering Laboratory, Department of Rheumatology and Clinical Immunology, Charité-University Medicine, Berlin 10117, Germany; Department of Otorhinolaryngology, Charité-University Medicine, Berlin 10117, Germany
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Abstract  The use of periosteum-derived progenitor cells (PCs) combined with bioresorbable materials is an attractive approach for tissue engineering. The aim of this study was to characterize the osteogenic differentiation of PC in 3-dimensional (3D) poly-lactic-co-glycolic acid (PLGA) fleeces cultured in medium containing allogeneic human serum. PCs were isolated and expanded in monolayer culture. Expanded cells of passage 3 were seeded into PLGA constructs and cultured in osteogenic medium for a maximum period of 28 d. Morphological, histological and cell viability analyses of three-dimensionally cultured PCs were performed to elucidate osseous synthesis and deposition of a calcified matrix. Furthermore, the mRNA expression of type I collagen, osteocalcin and osteonectin was semi-quantitively evaluated by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The fibrin gel immobilization technique provided homogeneous PCs distribution in 3D PLGA constructs. Live-dead staining indicated a high viability rate of PCs inside the PLGA scaffolds. Secreted nodules of neo-bone tissue formation and the presence of matrix mineralization were confirmed by positive von Kossa staining. The osteogenic differentiation of PCs was further demonstrated by the detection of type I collagen, osteocalcin and osteonectin gene expression. The results of this study support the concept that this tissue engineering method presents a promising method for creation of new bone in vivo.

Key words： Tissue engineering      Poly-lactic-co-glycolic acid polymer      Periosteum-derived progenitor cells      3-dimensional culture     
Received: 15 November 2005     
CLC:  Q819  
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ZHENG Yi-xiong
RINGE Jochen
LIANG Zhong
LOCH Alexander
CHEN Li
SITTINGER Michael
Cite this article:

ZHENG Yi-xiong, RINGE Jochen, LIANG Zhong, LOCH Alexander, CHEN Li, SITTINGER Michael. Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(10): 6-.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2006.B0817     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2006/V7/I10/6

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