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Study of cellulolytic soil fungi and two nova species and new medium |
KHALID Mahmood, YANG Wei-jun, KISHWAR Nazir, RAJPUT Zahid Iqbal, ARIJO Abdullah G. |
Government College, Satellite Town, Rawalpindi 46000, Pakistan; School of Life Sciences, Zhejiang University, Hangzhou 310058, China; Natural History Museum, Islamabad 44000, Pakistan; R & D Section, Directorate of Animal Husbandry Sindh, Shahbaz Building, Hyderabad 71000, Pakistan; Department of Parasitology, Sindh Agriculture University, Tando Jam 70060, Pakistan |
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Abstract This study is aimed at identifying and determining the percentage of occurrence frequency of cellulose decomposing soil fungi. The soil samples were inoculated into culture plates prepared in Sabouraud medium under sterilized conditions and incubated at 30 °C for 4 to 7 d. The identified fungal species were incubated in self-designed cellulose medium for testing their cellulolytic ability. Forty-two species, including 2 nova species, representing sixteen genera showed growth and sporulation in the cellulose medium. Most of the isolated species were from genus Aspergillus and Penicillium. Aspergillus niger and Mucor hiemalis showed highest occurrence frequency (45% and 36% respectively), as these species were collected from about 80% of soil samples. Being agar free and cheaper, the new fungal medium designed showed results equivalent to Sabouraud medium.
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Received: 17 March 2006
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