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Rapid in vitro propagation of medicinally important Aquilaria agallocha |
HE Meng-ling, QI Shu-yuan, HU Lan-juan |
South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou 510650, China; Chinese Traditional Medicine Department, Guangdong Pharmaceutical University, South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou 510650, China |
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Abstract Aquilaria agallocha can produce fragrant agarwood used for incense, traditional medicine and other products. An efficient plant regeneration system was established via organogenesis from shoots developed from seedlings of Aquilaria agallocha. Shoots generated many buds on MS medium supplemented with 1.3 μmol/L BA (6-benzylaminopurine) in the first 7 weeks, and the buds elongated on MS medium with 1.3 μmol/L BA+0.5 μmol/L NAA (naphthaleneacetic acid) in another 7 weeks, 2.3 shoots 2 cm in length per explant were obtained within 14 weeks. Plantlets were rooted on 1/2 MS medium after being immersed in 5 μmol/L NAA for 48 h, 96.7% of the roots grew up two weeks later. All plantlets that survived acclimatization grew well in the pots.
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Received: 26 January 2005
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