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Materials & Chemical Engineering
Improving the sensitivity of protein microarray by evanescent-field-induced fluorescence
WANG Li-qiang, LU Zu-kang
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2005, 6(7): 623-626.   https://doi.org/10.1631/jzus.2005.A0623
Abstract   PDF (0KB)
To improve the sensitivity of protein microarray, a prism surface replaces the surface of the common microscope slide. The protein targets arrayed on the surface are hybridized and labelled by fluorescent probes. Evanescent excitation occurs when the convergent laser reaches the surface, and a photomultiplier tube detects the emitted fluorescent signal. A two-dimensional actuator scans the whole surface to achieve planar laser excitation and fluorescence collection. The penetration depth of the evanescent field into the protein targets is only some hundred nanometers and can be controlled by different incident angle of the laser beam, so the undesired background signals are reduced dramatically and the detection sensitivity is improved by a factor of 50 to 100 comparing to confocal excitation. This approach can detect low abundance analytes without signal amplification.
Novel stereoselective sulfur ylide epoxidation reaction catalyzed by ferrocenylsulfide
WANG Lei, HUANG Zhi-zhen
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2005, 6(7): 636-639.   https://doi.org/10.1631/jzus.2005.A0636
Abstract   PDF (0KB)
A range of ferrocenyl sulfides are synthesized and screened. Among them 1-a-methysulphoferrocenyl ethyl acetate and 1-a-methysulphoferrocenyl alcohol are found to be unexpected catalysts, which is first reported mediating in sulfur ylide epoxidation reactions, furnishing a novel approach for highly stereoselective synthesis of oxiranes with 98%~100% trans-isomer. The protocol also has excellent yield, convenient workup and recycled starting material. The reason of high trans-selectivity is due to the bulky ferrocenyl sulfide group, which stabilizes the intermediates and determines the trans priority. A possible catalytic mechanism is also proposed.
Discovery of ferromanganese crust boundary and its genetic and ore prospecting significance
CHU Feng-you, QIAN Xin-yan, ZHANG Hai-sheng, MA Wei-lin, JIN Xiang-long, SUN Guo-sheng
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2005, 6(7): 656-662.   https://doi.org/10.1631/jzus.2005.A0656
Abstract   PDF (0KB)
Evidences for the existence of ferromanganese crust boundary were found for the first time during the survey of the “DA YANG YI HAO” Vessel 2003. Some typical characteristics of the boundary are summarized and the significances of the finding of the boundary are included in the genesis discussion. Ore prospecting and assessment of the crust resources are described in this paper. The morphologic and extending characteristics of the crust boundary led to the recognition of two types of crust boundary: interpenetration crust boundary and closed crust boundary. According to the distribution and types of the crust, however, the boundaries are classified into three types: the boundary between ferromanganese crust and detrital sediment, the boundary between tabular crust and seamount nodules, and the boundary between tabular crust and rudaceous crust. This study revealed that the boundary between tabular crust and nodules was not formed under different regional environments but formed under different nucleation potential barriers between different initial growth (nucleation and germination) processes and between solid rocks and loose sediments. The rudaceous crusts are controlled spatially by fracture zones and the crusts’ boundaries are controlled by the seamount structure and landform. The discovery of the crust boundaries reveals the crust’s ‘negative growth’ phenomena (especially for some seamount nodules). The boundary investigation can be helpful in identifying the existence of tabular crust covered by detrital sediments and in calculating the area covered by the crust. The resource calculation error and the resource quality as well as resource exploration degree can be assessed through the survey of crust boundary.
Civil & Mechanical Engineering
Heat transfer characteristics of air cross-flow for in-line arrangement of spirally corrugated tube and smooth tube bundles
LU Guo-dong, ZHOU Qiang-tai, TIAN Mao-cheng, CHENG Lin, YU Xiao-li
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2005, 6(7): 670-675.   https://doi.org/10.1631/jzus.2005.A0670
Abstract   PDF (0KB)
An experimental study on heat transfer and resistance coefficients of linearly arranged smooth and spirally corrugated tube bundles in cross-flow was performed. The heat transfer and resistance coefficients are presented in this paper with transverse and longitudinal tube-pitch and tube geometries taken into account. The experiment’s results can provide technical guidelines for application to horizontal air preheater with arranged in-line spirally corrugated tube bundles, especially to the air preheater for CFBCBs (Circulating Fluidized Bed Combustion Boilers).
Computer & Information Science
Cooperative co-evolution based distributed path planning of multiple mobile robots
WANG Mei, WU Tie-jun
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2005, 6(7): 697-706.   https://doi.org/10.1631/jzus.2005.A0697
Abstract   PDF (0KB)
This paper proposes novel multiple-mobile-robot collision avoidance path planning based on cooperative co-evolution, which can be executed fully distributed and in parallel. A real valued co-evolutionary algorithm is developed to coordinate the movement of multiple robots in 2D world, avoiding C-space or grid net searching. The collision avoidance is achieved by cooperatively co-evolving segments of paths and the time interval to pass them. Methods for constraint handling, which are developed for evolutionary algorithm, make the path planning easier. The effectiveness of the algorithm is demonstrated on a number of 2D path planning problems.
Reconstruction algorithm in lattice-invariant signal spaces
XIAN Jun
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2005, 6(7): 760-763.   https://doi.org/10.1631/jzus.2005.A0760
Abstract   PDF (0KB)
In this paper, we mainly pay attention to the weighted sampling and reconstruction algorithm in lattice-invariant signal spaces. We give the reconstruction formula in lattice-invariant signal spaces, which is a generalization of former results in shift-invariant signal spaces. That is, we generalize and improve Aldroubi, Gröchenig and Chen’s results, respectively. So we obtain a general reconstruction algorithm in lattice-invariant signal spaces, which the signal spaces is sufficiently large to accommodate a large number of possible models. They are maybe useful for signal processing and communication theory.
Analysis on management of philanthropic endowment in Chinese universities
MENG Dong-jun, WU Ying-jun, ZHANG Mei-feng
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2005, 6(7): 775-778.   https://doi.org/10.1631/jzus.2005.A0775
Abstract   PDF (0KB)
Philanthropic endowment, along with government allocation, tuition fee and extra income, is one of the four major means for funding higher education in China. Research on philanthropic endowment is at its beginning stage, and so it is not yet well known. With a view to providing some useful information to our government, universities and society, this paper aims at making a brief analysis of the status quo and management of philanthropic endowment in Chinese universities.
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