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Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  0, Vol. 7 Issue (101): 366-370    DOI: 10.1631/jzus.2006.AS0366
Biotechnological Engineering     
Improvement of spinosad producing Saccharopolyspora spinosa by rational screening
Jin Zhi-Hua, Wu Jian-Ping, Zhang Yuan, Cheng Xiu, Yang Li-Rong, Cen Pei-Lin
Department of Biological and Pharmaceutical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China; Institute of Bioengineering, School of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China
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Abstract  Spinosad (spinosyns A and D) is a mixture of secondary metabolites produced by Saccharopolyspora spinosa. It is used in agriculture as a potent insect control agent with exceptional safety to non-target organisms. Spinosyns are macrolides with a 21-carbon, tetracyclic lactone backbone to which the deoxysugars forosamine and tri-O-methylrhamnose are attached. According to the pathway and regulation of spinosad biosynthesis, a rational selection procedure with u.v. mutation was performed to obtain high spinosad producing strain. The spinosad resistant mutants, the rhamnose resistant mutants, the 2-deoxygen-D-glucose resistant mutants were selected, successively. Eventually, a strain S. spinosa 4~6 was obtained, with its production of spinosad reaching 268 mg/L, which is increased by 121% in comparison with that of the parent strain S. spinosa 1~5. The subculture experiments indicated that the hereditary character of high production of S. spinosa 4~6 is stable. The spinosad fermentation with S. spinosa 4~6 was scaled up in a 10 L fermentor, and a production of 458 mg/L was obtained, which was 71% higher than the production with shaking-flask fermentation.

Key wordsSpinosad      Saccharopolyspora spinosa      Strain improvement      Rational screening     
Received: 01 November 2005     
CLC:  Q815  
Cite this article:

Jin Zhi-Hua, Wu Jian-Ping, Zhang Yuan, Cheng Xiu, Yang Li-Rong, Cen Pei-Lin. Improvement of spinosad producing Saccharopolyspora spinosa by rational screening. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 0, 7(101): 366-370.

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http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2006.AS0366     OR     http://www.zjujournals.com/xueshu/zjus-a/Y0/V7/I101/366

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[2] Jin Zhi-Hua, Wu Jian-Ping, Zhang Yuan, Cheng Xiu, Yang Li-Rong, Cen Pei-Lin. Improvement of spinosad producing Saccharopolyspora spinosa by rational screening[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2006, 7(Supplement2): 366-370.