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ZJUS-A
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2004, Vol. 5 Issue (12): 1608-1612    DOI: 10.1631/jzus.2004.1608
Biotechnology     
Characterization of cellulose acetate micropore membrane immobilized acylase I
GUO Yong-sheng, WANG Jie, SONG Xi-jin
Department of Chemistry, Zhejiang University, Hangzhou 310027, China
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Abstract  This paper describes an innovative method for the immobilization of acylase I, which was entrapped into the CA-CTA micropore membrane. The most suitable casting solutions proportion for immobilizing the enzyme was obtained through orthogonal experiment. Properties of the enzyme membrane were investigated and compared with those of free enzyme and blank membrane. The thermal stability and pH stability of the enzyme inside the membrane were changed by immobilization. The optimum pH was found to be 6.0, which changes 1.0 unit compared with that of free acylase I. The optimum temperature was found to be about 90 °C, which is higher than that of free acylase I (60 °C). Experimental results showed that immobilization had effects on the kinetic parameters of acylase I.

Key words： Acylase I      CA-CTA micropore membrane      Enzyme immobilization      Orthogonal experiment     
Received: 21 November 2003     
CLC:  Q55  
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WANG Jie
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Cite this article:

GUO Yong-sheng, WANG Jie, SONG Xi-jin. Characterization of cellulose acetate micropore membrane immobilized acylase I. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2004, 5(12): 1608-1612.

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http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2004.1608     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2004/V5/I12/1608

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