IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS
CHEN Zhi, LIU Yong, LIU Ke-zhou, Dennin R.H, Reinhard U, Dou Jun
Institute of Infectious Diseases, Medical College, Zhejiang University, Hangzhou 310003, China; Institute of Microbiology, Lubeck Medical University, Germany
Abstract Objective: To determine the effective nucleotide sites of ribozymes against HCV, and obtain a highly effective, nontoxic and inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5\'NC region and C region, were designed and synthesized.Eukaryotic expression vectors, pSV2-gpt. CD-SR, containing either HCRZNC or HCRZC were constructed and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hybridization were used to determine the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 2.1%, respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion: Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better effect when used together.
CHEN Zhi, LIU Yong, LIU Ke-zhou, Dennin R.H, Reinhard U, Dou Jun. IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2001, 2(1): 100-103.
