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ZJUS-A
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2002, Vol. 3 Issue (5): 563-566    DOI: 10.1631/jzus.2002.0563
Chemistry & Material Science     
Fiber optic biosensor of immobilized firefly luciferase
CAI Jin, MENG Wen-fang, JI Xin-song
College of Material & Chemical Engineering, Zhejiang University, Hangzhou 310027, China; Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, China; Institute of Biochemistry and Cell Biology, The Chinese Academy of Sciences, Shanghai 200031, China
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Abstract  Luciferase from firefly lantern extract was immobilized on CNBr-activated Sepharose 4B. The kinetic properties of immobilized luciferase were extensively studied. The Km′ for D-luciferin is 11.9 μmol/L, the optimum pH and temperature for Sepharose-bound enzyme were 7.8 and 25°C respectively. A luminescence fiber optic biosensor, making use of immobilized crude luciferase, was developed for assay of ATP. The peak light intensity was linear with respect to ATP concentration in range of 10-9–10-5 mol/L. A biological application was also demonstrated with the determination of serum ATP from rats bred in low versus normal oxygen environments.

Key words： Firefly luciferase      ATP      Immobilized enzyme      Fiber optic biosensor     
Received: 06 March 2002     
CLC:  Q814.3  
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CAI Jin
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JI Xin-song
Cite this article:

CAI Jin, MENG Wen-fang, JI Xin-song. Fiber optic biosensor of immobilized firefly luciferase. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2002, 3(5): 563-566.

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http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2002.0563     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2002/V3/I5/563

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