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ZJUS-A
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2002, Vol. 3 Issue (3): 348-354    DOI: 10.1631/jzus.2002.0348
Life Sciences & Biotechnology     
Analysis of bulked segregants to identify molecular markers linked with cocoon weight and cocoon shell weight in the silkworm Bombyx mori L
Sateesh Kumar, XU Meng-kui, CHEN Yu-yin, Ponnuvel, K.M., Datta, R.K.
Department of Sericulture & Apiculture, Zhejiang University, Hangzhou 310029, China; Molecular Biology Laboratory, Central Sericultural Research and Training Institut Mysore 570008, India
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Abstract  Two silkworm strains viz, B20 A (high cocoon shell ratio) and C.Nichi (low cocoon shell ratio) were sib mated for 10 generations to determine the homozygosis. Both bulked segregant analysis(BSA) and near isogenic lines (NIL) studies were done to identify the RFLP markers closely linked to cocoon shell parameters. Three hundred and fifty-two random clones were identified as the low copy number sequence and used for identification of Restriction Fragment Length Polymorphic (RFLP) marker linked to cocoon weight and cocoon shell character. In the bulk segregant analysis, DNA from the parents (B20 A, C.Nichi), F1 and F2 progeny of high shell ratio (HSR) and low shell ratio (LSR) were screened for hybridization with the random clones. Polymorphic banding pattern achieved through southern hybridization with different probes indicated the probable correlation of polymorphism with high and low cocoon shell character which are possible landmarks in identifying the putative marker(s) for the cocoon shell character. Out of the 100 probes tried with parents, F1, F2 and their bulks, 10 probes were found to be closely linked to cocoon shell characters.

Key words： Restriction fragment length polymorphic (RFLP)      Molecular marker      Bombyx mori L      Shell ratio      Bulked segregant analysis (BSA)      Near isogenic lines     
Received: 29 December 2001     
CLC:  Q819  
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Sateesh Kumar
XU Meng-kui
CHEN Yu-yin
Ponnuvel
K.M.
Datta
R.K.
Cite this article:

Sateesh Kumar, XU Meng-kui, CHEN Yu-yin, Ponnuvel, K.M., Datta, R.K.. Analysis of bulked segregants to identify molecular markers linked with cocoon weight and cocoon shell weight in the silkworm Bombyx mori L. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2002, 3(3): 348-354.

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http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2002.0348     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2002/V3/I3/348

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