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ZJUS-A
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2004, Vol. 5 Issue (8): 936-940    DOI: 10.1631/jzus.2004.0936
Materials & Chemical Science     
Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method
JIANG Bing-bing, GAO Chang-you, HU Ling, SHEN Jia-cong
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China
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Abstract  A modified complex coacervation-co-precipitation method was used to prepare bone morphogenetic protein (BMP)-loaded nanospheres. Three natural polymers were used as packing materials to obtain nanoscale delivery device for BMP, in the presence of phosphatidylcholine functioning as stabilizer. Positively charged polysaccharide, N,N-diethylaminoethyl dextran (DEAE-dextran) tended to form stable, uniform and smaller size particles carrying BMP. Negatively charged bovine serum al-bumin (BSA) induced precipitation of the produced BMP particles due to its weak interaction with BMP molecules, although it produced nanosized BMP spheres. While collagen, a weakly positively charged protein shaped larger particles due to the strong interaction among themselves. A mechanism of co-precipitation process was also deduced to depict the formation of stable nanospheres.

Key words： Co-precipitation      Nanoparticles      Bone morphogenetic proteins      Biopolymers     
Received: 28 September 2003     
CLC:  O630  
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JIANG Bing-bing
GAO Chang-you
HU Ling
SHEN Jia-cong
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JIANG Bing-bing, GAO Chang-you, HU Ling, SHEN Jia-cong. Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2004, 5(8): 936-940.

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http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2004.0936     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2004/V5/I8/936

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