| Biomedical Science |
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| Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor |
| YANG Yun-mei, CAO Hong-cui, XU Zhe-rong, CHEN Xiao-ming |
| First Affiliated Hospital, College of Medical Science, Zhejiang University, Hangzhou 310003, China |
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Abstract Objective: To establish a new assay for platelet-activating factor (PAF), to compare it with bio-assay; and to discuss its significance in some elderly people diseases such as cerebral infarction and coronary heart disease. Methods: To measure PAF levels in 100 controls, 23 elderly patients with cerebral infarction and 65 cases with coronary heart disease by reversed phase high-performance liquid chromatographic technique (rHPLC). Results: rHPLC is more convenient, sensitive, specific, and less confusing, compared with bio-assay. The level of plasma PAF in patients with cerebral infarction was higher than that in the controls (P<0.01), and in patients with coronary heart disease. Conclusion: Detection of PAF with rHPLC is more reliable and more accurate. The new assay has important significance in PAF research.
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Received: 10 June 2003
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