Please wait a minute...
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2004, Vol. 5 Issue (2): 222-225    DOI: 10.1631/jzus.2004.0222
Biomedical Science     
Identification of genes differentially expressed in monocyte-derived dendritic cells with 1α,25-dihydroxyvitamin D3 using cDNA arrays
SHEN Qian-yun, ZHENG Shu-sen
Department of Surgery, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China
Download:     PDF (0 KB)     
Export: BibTeX | EndNote (RIS)      

Abstract  In order to study the molecular mechanism of the inhibitory effect of 1,25-dihydroxyvitamin D3 on dendritic cells, experiments were performed using Atlas cDNA expression arrays from Clonetech to identify the differentially expressed genes of dendritic cells by 1,25-dihydroxyvitamin D3. Analysis of cDNA arrays revealed changes in the expression of 9 genes, including those involved in DNA binding and transcription, extracellular cell signaling and communication, intracellular transducers, as well as cell adhesions. The results indicated that a multiple molecular network is involved in the inhibitory role of 1,25-dihydroxyvitamin D3 on dendritic cells. The Atlas Array technology may facilitate the elucidation of complex pharmacological process of 1,25-dihydroxyvitamin D3 on dendritic cells.

Key words1,25-dihydroxyvitamin D3      Dendritic cells      Gene expression      cDNA array     
Received: 27 February 2003     
CLC:  TP391.72  
Cite this article:

SHEN Qian-yun, ZHENG Shu-sen. Identification of genes differentially expressed in monocyte-derived dendritic cells with 1α,25-dihydroxyvitamin D3 using cDNA arrays. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2004, 5(2): 222-225.

URL:

http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2004.0222     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2004/V5/I2/222

[1] Danial Behnia, Kaveh Ahangari, Ali Noorzad, Sayed Rahim Moeinossadat. Predicting crest settlement in concrete face rockfill dams using adaptive neuro-fuzzy inference system and gene expression programming intelligent methods[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2013, 14(8): 589-602.
[2] HUANG Xian-mei, ZHU Wei-hua, KANG Man-li. Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca2+ transport proteins and the effect of taurine on myocardial protection in rabbits[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2003, 4(1): 114-120.