Please wait a minute...
ZJUS-A
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2004, Vol. 5 Issue (2): 157-163    DOI: 10.1631/jzus.2004.0157
Bioscience & Biotechnology     
Ex vivo expansions and transplantations of mouse bone marrow-derived hematopoietic stem/progenitor cells
WANG Jin-fu, WU Yi-fan, HARRINTONG Jenny, McNIECE Ian K.
College of Life Sciences, Zhejiang University, Hangzhou 310012, China; BMT Lab, University of Colorado Health Science Center, Denver, CO 80262, USA
Download:     PDF (0 KB)     
Export: BibTeX | EndNote (RIS)      

Abstract  To examine the effects of co-culture with bone marrow mesenchymal stem cells on expansion of hematopoietic stem/progenitor cells and the capacities of rapid neutrophil engraftment and hematopoietic reconstitution of the expanded cells, we expanded mononuclear cells (MNCs) and CD34+/c-kit+ cells from mouse bone marrow and transplanted the expanded cells into the irradiated mice. MNCs were isolated from mouse bone marrow and CD34+/c-kit+ cells were selected from MNCs by using MoFlo Cell Sorter. MNCs and CD34+/c-kit+ cells were co-cultured with mouse bone marrow-derived mesenchymal stem cells (MSCs) under a two-step expansion. The expanded cells were then transplanted into sublethally irradiated BDF1 mice. Results showed that the co-culture with MSCs resulted in expansions of median total nucleated cells, CD34+ cells, GM-CFC and HPP-CFC respectively by 10.8-, 4.8-, 65.9- and 38.8-fold for the mononuclear cell culture, and respectively by 76.1-, 2.9-, 71.7- and 51.8-fold for the CD34+/c-kit+ cell culture. The expanded cells could rapidly engraft in the sublethally irradiated mice and reconstitute their hematopoiesis. Co-cultures with MSCs in conjunction with two-step expansion increased expansions of total nucleated cells, GM-CFC and HPP-CFC, which led us to conclude MSCs may create favorable environment for expansions of hematopoietic stem/progenitor cells. The availability of increased numbers of expanded cells by the co-culture with MSCs may result in more rapid engraftment of neutrophils following infusion to transplant recipients.

Key words： Hematopoietic stem cells      Mesenchymal stem cells      Expansion      Transplantation     
Received: 09 December 2002     
CLC:  R239.28  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
HARRINTONG Jenny
McNIECE Ian K.
WANG Jin-fu
WU Yi-fan
Cite this article:

WANG Jin-fu, WU Yi-fan, HARRINTONG Jenny, McNIECE Ian K.. Ex vivo expansions and transplantations of mouse bone marrow-derived hematopoietic stem/progenitor cells. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2004, 5(2): 157-163.

URL:

http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2004.0157     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2004/V5/I2/157

[1] Xiao-wei Zhang, Zhi-feng Tang, Fu-zai Lv, Xiao-hong Pan. Excitation of axisymmetric and non-axisymmetric guided waves in elastic hollow cylinders by magnetostrictive transducers[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2016, 17(3): 215-229.
[2] Ke Tang, Long Sha, Yi-jian Li, Tao Jin, Shu-juan Liu. Measurement of thermal expansion at low temperatures using the strain gage method[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2014, 15(5): 323-330.
[3] Jun-hua CHEN, Yi-jie WU. Research in non-equalization machining method for spatial cam[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2008, 9(9): 1258-1263.
[4] LUO Zhan-you, ZHU Xiang-rong, GONG Xiao-nan. Expansion of spherical cavity of strain-softening materials with different elastic moduli of tension and compression[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2007, 8(9): 1380-1387.
[5] CHEN Jun-hua, WU Yi-jie. Analysis of 3D curve expansion of conical cam with oscillating tapered roller follower[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2007, 8(11): 1789-1794.
[6] Hu Jun, Yan Chun-sheng, Lin Qing-chun. A new patch antenna with metamaterial cover[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2006, 7(1): 89-94.
[7] WANG Hui-jin, HU Hua, LI Qing. A dynamic knowledge base based search engine[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2005, 6( 7): 13-.
[8] LIN Qing-chun, ZHU Fang-ming, HE Sai-ling. A new photonic bandgap cover for a patch antenna with a photonic bandgap substrate[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2004, 5(3): 269-273.
[9] WANG Jian-an, LI Chang-ling, FAN You-qi, HE Hong, SUN Yong. Allograftic bone marrow-derived mesenchymal stem cells transplanted into heart infarcted model of rabbit to renovate infarcted heart[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2004, 5(10): 1279-1285.
[10] JIA Chang-ku, ZHENG Shu-sen, ZHANG Ai-bin. Intrathymic inoculation of donor liver specific antigen alleviates rejection of liver allotransplantation[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2003, 4(4): 485-490.
[11] HUANG Dong-sheng, ZHENG Shu-sen, WU Jian, LIANG Ting-bo, WANG Wei-ling, SHEN Yan, ZHANG Min. Evaluation of orthotopic liver transplantation with no veno-venous bypass[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2002, 3(4): 480-483.
[12] YUE Lin-hai, SHUI Miao, ZHU Wang-liang, XU Zhu-de. PHOTOCATALYTIC ACTIVITY OF LANTHANUM DOPING TiO2[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2001, 2(3): 271-274.