| Materials Science & Chemical Engineering |
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| Reuse of waste frying oil for production of rhamnolipids using Pseudomonas aeruginosa zju.u1M |
| ZHU Yong, GAN Jun-jiang, ZHANG Guo-liang, YAO Bin, ZHU Wen-jie, MENG Qin |
| Shool of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China; School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310032, China; Institute of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China |
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Abstract In this work, rhamnolipid production was investigated using waste frying oil as the sole carbon source. By culture in shaking flasks, a naturally isolated strain synthesized rhamnolipid at concentration of 12.47 g/L and its mutant after treatment by UV light increased this productivity to 24.61 g/L. Fermentation was also conducted in a 50 L bioreactor and the productivity reached over 20 g/L. Hence, with a stable and high productive mutant strain, it could be feasible to reuse waste frying oil for rhamnolipid production on industrial scale.
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Received: 20 April 2006
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