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ZJUS-A
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2007, Vol. 8 Issue (2 ): 1-    DOI: 10.1631/jzus.2007.A0171
    
Capillary method for measuring near-infrared spectra of microlitre volume liquids
YUAN Bo, MURAYAMA Koichi
Department of Information Science and Optoelectronic Engineering, State Key Laboratory of Modern Optical Instrumentation, CNERC for Optical Instrument, Zhejiang University, Hangzhou 310027, China; Department of Biochemistry and Biophysics, Gifu University School of Medicine, Gifu 500-8705, Japan
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Abstract  The present study theoretically explored the feasibility of the capillary method for measuring near-infrared (NIR) spectra of liquid or solution samples with microlitre volume, which was proposed in our previous studies. Lambert-Beer absorbance rule was applied to establish a model for the integral absorbance of capillary, which was then implemented in numerical analyses of the effects of capillary on various spectral features and dynamic range of absorption measurement. The theoretical speculations indicated that the capillary method might be used in NIR spectroscopy, which was further supported by the empirical data collected from our experiments by comparison between capillary NIR spectra of several organic solvents and cuvette cell NIR spectra.

Key words： Near-infrared (NIR) spectroscopy      Capillary      Cuvette cell      Numerical analysis     
Received: 17 March 2006     
CLC:  O43  
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YUAN Bo
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YUAN Bo, MURAYAMA Koichi. Capillary method for measuring near-infrared spectra of microlitre volume liquids. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2007, 8(2 ): 1-.

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http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2007.A0171     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2007/V8/I2 /1

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