| Bioscience & Biotechnology |
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| A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor |
| LI Yong-quan, CEN Pei-lin, CHEN Shi-fei, WU Dan, ZHENG Jing |
| College of Life Science, Zhejiang University, Hongzhou 310027, China; Institute of Bioengineering, Zhejiang University, Hongzhou 310027, China; Drug Adiministration of Zhejiang Province, Hongzhou 310012, China |
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Abstract Two-component genes are kinds of genetic elements involved in regulation of antibiotic production in Streptomyces coelicolor. DNA microarray analysis revealed thatecr A1/A2, which mapped at distant sites from red locus and encode respectively the kinase and regulator, expressed coordinately with genes of Red specific biosynthetic pathway. ecrA1 and ecrA2 gene-disruptive mutants were constructed using homogenotisation by reciprocal double crossover. Fermentation data showed that the undecylprodigiosin (Red) level of production was lower than that of wild-type strain. However, the change of the actinorhodin (Act) production level was not significant compared with wild type. Thus, these experiment results confirmed that the two-component system ecrA1/A2 was positive regulatory element for red gene cluster.
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Received: 24 January 2003
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