Abstract: In this paper, the hemoglobin(Hb) and serum protein were analysed by means of thin-Iayer isoelec-tric focusing, while the serum protein was analysed using the method of SDS-PAGE, in order to findthe biochemical characteristics and their variations of the Musk Shrews from different geographical pop-ulation,trapped from Xiaoshan,Jiemei (continent) and Zhoushan(island).The results were as follows] 1.There were 6 bands of Hb in continent population. However,4 types are found in island popula-tion.2.Thereand 13were significantdifference on the number of bands (23 bands in Xiaoshan, 22 bands inJiemeibands in Zhoushan) and their stained colour (darkest in Jiemei) of the serum 3. With the SDS-PAGE method, there were 38 bands of the serum protein in Xianshanand 37 bands in Zhoushan and Jiemei population.protein.population