Abstract：L336 can produce high yield acid protease. By replacement culture method, some kinetic characters of its fermentation process w ere studied. The time course of this process manifests a distinct feature w hich belongs to the type non-grow th-associated product formation, and the acid protease producing activity emerges when grow th is ceased due to the exhaustion of adenine-group
grow th substance. The enzyme synthesis will be interrupted by inhibitors such as 8-azaguanine and 8azaadenine, and will be increased by increasing oxygen concerntration in fermental liquid. These facts illustrated the De Novo syntheses of the enzyme protein. The effect of peptone ininducing the enzyme is much more remarkable, protein hydrolyate such as bean cake and silkw orm chrysalis are also more effective in inducing the enzyme. All the results above lay the theoretical foundation of fermentation technique.