Abstract：Nitrobacteria DNA was extracted from wastewater of biological nitrification stage and two PCR primers based on the DNA sequences of 16S r DNA and 23S r DNA were synthesized and used for PCR amplification. The results showed that Nitrobacteria DNA was amplified specifically and the size of amplified DNA fragments were about 400 bp and 730 bp respectively. It is suggested that PCR analysis can be used for the rapid detection of Nitrobacteria in wastewater.
