Abstract： Rat embryonic cerebral neurocytes(RECN) were cryopreserved by vitrification at-
196℃. The samples were separately thawed after storage for 1、10、20 and 40 days,and then
cryoprotective solution was diluted and removed. RECN were assayed for their viability and
function,and were transplanted for spinal cord injury in rats. The results showed that RECN
cryopreserved by vitrification for 40 days retained survival rate of 76.4±8.3％. Compared with
the fresh neurocytes,the cell viabiliry and membrane integrity of the frozen-thawed RECN were
decreased,but they still maintained at relatively high level.Synaptic connection was established in
neuron culture for 1 week. Cryopreserved RECN were transplanted for spinal cord injury in rats,
there was some recovery both in sensation and movement function. No obvious difference was
found between vitrification and slow cooling group.
胡军祥，姜玉新，周晴，郑斯涌，曾跃武，冯春木. 玻璃化冻存对胚胎神经细胞活性及脊髓移植的影响[J]. 浙江大学学报（理学版）, 1999, 26(1): 65-69.
Hu Junxiang，Jiang Yuxin，Zhou Qing，Zheng Siyong. Effect of Vitrified Cryopreservation on Embryonic Neurocyte Viability and Transplantation for Spinal Cord Injury in Rats. Journal of ZheJIang University(Science Edition), 1999, 26(1): 65-69.