Effect of silver nanoparticles on biofilm formation of Pseudomonas fluourcens
HUANG Xunchen1,2, YANG Wei1,2, DAI Xianjun1,2
1. College of Life Sciences, China Jiliang University, Hangzhou 310018, China;
2. Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province, Hangzhou 310018, China
Abstract：To explore the effect of silver nanoparticles on the formation of Pseudomonas fluourcens biofilms on ethylene-vinyl acetate(EVA) pieces, the curve of bacterial biofilm is determined by colony counts, and the morphology, microstructure and bacterial status of the biofilm are respectively observed through crystal voilet staining, scanning electron microscopy(SEM) and confocal laser scanning microscope(CLSM).The biofilm stained by crystal violet is examined with a digital microscope to calculate the biofilm coverage rate (BCR) by NIS-Element BR soft ware. Then the mortality of bacterial is calculated by image structure analyzer (ISA) software.Results show that the bacteria can aggregate on the control EVA and EVA contained 0.4% silver nanoparticles, and forms the grown biofilm after 12 and 48 h culture, respectively. But the biofilm on the control EVA has more bacterial count and BCR than that of silver nanoparticles(p<0.05). At 24 h, the bacteria interacting with its extracellular products can form the dense film in the microscope vision of 10000 times on the control EVA, while there are only dispersive bacteria on silver nanoparticle EVA. The bacterial mortality in the film on silver nanoparticle EVA is significantly higher than that of control group at 24, 48 h (p<0.05). Silver nanoparticles have great inhibiting effect on biofilm formation of Pseudomonas fluourcens through inhibiting the growth of the bacterial and its extracellucar products.
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2016, Vol. 43 
