Please wait a minute...
浙江大学学报(理学版)  2016, Vol. 43 Issue (6): 751-755    DOI: 10.3785/j.issn.1008-9497.2016.06.022
生命科学     
铬胁迫对西红花叶片蛋白表达谱的影响
饶君凤1, 吕伟德1, 曹方彬2
1. 杭州职业技术学院, 浙江 杭州 310018;
2. 浙江大学 农业与生物技术学院, 浙江 杭州 310058
Effect of chromium stress on protein profiles in saffron
RAO Junfeng1, LYU Weide1, CAO Fangbin2
1. Hangzhou Vocational & Technical College, Hangzhou 310018, China;
2. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
 全文: PDF(1085 KB)  
摘要: 铬是植物的非必需元素,对植物的生长发育具有显著的抑制作用.采用双向电泳和质谱技术,研究了铬胁迫对西红花叶片蛋白表达的影响.通过质谱技术,成功鉴定出9个在铬胁迫后下调表达的蛋白,分别为细胞分裂循环蛋白48、ATP合成酶α亚基、核酮糖二磷酸羧化酶长链(2个)、未知蛋白、核酮糖二磷酸羧化加氧酶、蛋白酶体α亚基、铁蛋白和蛋白酶体β亚基;6个上调表达的蛋白,分别为蔗糖合酶、真核起始因子4A、α-1,4-葡聚糖蛋白合成酶、1-氨基环丙烷-1-羧酸氧化酶、异黄酮还原酶类似物IRL和未知蛋白.以上结果为研究植物响应铬胁迫的分子机制提供了参考.
关键词: 西红花叶片蛋白组    
Abstract: Chromium(Cr) is a nonessential element for plants. Cr not only affects a series of physiological processes in plants, but also significantly inhibits the growth and development of plants. Under Cr stress, plants have evolved complex detoxification mechanisms. In the present study, we investigated the protein expression of leaves of saffron in response to Cr stress via 2D electrophoresis and mass spectrum analysis. Nine proteins were identified to be down-regulated under Cr stress, including cell division cycle protein 48 homolog, ATP synthase subunit alpha, ribulose bisphosphate carboxylase large chain, Proteasome subunit alpha type, etc. Six proteins were up-regulated, including sucrose synthase 2, eukaryotic initiation factor 4A, Alpha-1,4-glucan-protein synthase, 1-aminocyclopropane-1-carboxylate oxidase and Isoflavone reductase homolog IRL, etc. The results provide valuable insights towards the molecular mechanism of saffron in response to Cr toxicity.
Key words: chromium    saffron    leaf    proteomics
收稿日期: 2015-11-02 出版日期: 2017-03-07
CLC:  R282.2  
基金资助: 科技部国家星火计划项目(2015GA700053);浙江省自然科学基金资助项目(LY13B020001);浙江省科技厅公益技术研究农业项目(2015C32104).
作者简介: 饶君凤(1969-),ORCID:http://orcid.org/0000-0000-0002-069X,女,副教授,主要从事中药品质评价和资源应用研究,E-mail:13588867756@139.com.
服务  
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章  
饶君凤
吕伟德
曹方彬

引用本文:

饶君凤, 吕伟德, 曹方彬. 铬胁迫对西红花叶片蛋白表达谱的影响[J]. 浙江大学学报(理学版), 2016, 43(6): 751-755.

RAO Junfeng, LYU Weide, CAO Fangbin. Effect of chromium stress on protein profiles in saffron. Journal of ZheJIang University(Science Edition), 2016, 43(6): 751-755.

链接本文:

https://www.zjujournals.com/sci/CN/10.3785/j.issn.1008-9497.2016.06.022        https://www.zjujournals.com/sci/CN/Y2016/V43/I6/751

[1] ZAYED A M, TERRY N. Chromium in the environment:Factors affecting biological remediation[J]. Plant and Soil,2003,249:139-156.
[2] 刘婉,李泽琴.水中铬污染治理的研究进展[J].广东微量元素科学,2008,14:5-9. LIU Wan, LI Zeqin. Development of governance of chromium pollution in water[J]. Guangzhou Trace Element Science,2008,14:5-9.
[3] PANDA S K, CHOUDHURY S. Chromium stress in plants[J]. Brazilian Journal of Plant Physiology,2005,17:95-102.
[4] 朱建华,王莉莉.不同价态铬的毒性及其对人类的影响[J].环境与开发,1997,12:46-48. ZHU Jianhua, WANG Lili. The toxicity of different valence chromium and effect on human body[J]. Environment and Exploitation, 1997,12:46-48.
[5] SHANKER A K, CERVANTES C, LOZA-TAVERA H, et al. Chromium toxicity in plants[J]. Environment International,2005,31:739-753.
[6] CAI Y, CAO F, WEI K, et al. Genotypic dependent effect of exogenous glutathione on Cd-induced changes in proteins, ultrastructure and antioxidant defense enzymes in rice seedlings[J]. Journal of Hazardous Materials, 2011,192:1056-1066.
[7] 吴德志.西藏野生大麦耐盐种质的发掘及其耐盐机制研究[D].杭州:浙江大学,2012. WU Dezhi. Exploration of Salt-Tolerant Germplasm and Its Mechanism in Tibetan Wild Barley[D]. Hangzhou:Zhejiang University, 2012.
[8] ZENG F, QIU B Y, WU X J, et al. Glutathione-mediated alleviation of chromium toxicity in rice plants[J]. Biological Trace Element Research, 2012,148:255-263.
[9] CAO F, WANG N, ZHANG M, et al. Comparative study of alleviating effects of GSH, Se and Zn under combined contamination of cadmium and chromium in rice(Oryza sativa)[J]. Biometals, 2013,26:297-308.
[10] MIYADATE H, ADACHI S, HIRAIZUMI A, et al. OsHMA3, a P1B-type of ATPase affects root-to-shoot cadmium translocation in rice by mediating efflux into vacuoles[J]. New Phytologist, 2011,189:190-199.
[11] 李运合,钱善勤,覃逸明.盐胁迫下外源NO对玉米幼苗叶片蔗糖代谢的影响[J].湖北农业科学,2010,49(7):1589-1592. LI Yunhe, QIAN Shanqin, QIN Yiming. Effect of exogenous nitric oxide on sucrose metabolism of maize seedlings leaves under salt stress[J]. Hubei Agricultural Sciences, 2010,49(7):1589-1592.
[12] HERBETTE S, TACONNAT L, HUGOUVIEUX V, et al. Genome-wide transcriptome profiling of the early cadmium response of Arabidopsis roots and shoots[J]. Biochimie, 2006,88:1751-1765.
[1] 饶君凤, 吕伟德, 宋涛. 微生物肥料对西红花生长及代谢物的影响[J]. 浙江大学学报(理学版), 2019, 46(6): 731-736.